Cetrimide Broth

Media
Sabouraud Dextrose Broth
Products
Sabouraud Dextrose Broth
The medium is used for the quantitative
identification of yeast and
mold.
Description
Peptone in the media is used as a
nitrogen source for the development of
fungi. Dextrose acts as an energy
source for the growth of microorganisms.
The low pH is favorable for
the development of fungi, especially
dermatophytes, but at the same time
inhibits the development of contaminating
bacteria from clinical specimens.
Interpretation:
Growth is interpreted as yeast/mold.
Subculturing and species/group specific
identification is required.
Quality Control and recommended
incubation conditions:
Positive control:
Candida albicans ATCC 10231
incubated for 72 hours at 25–30º C.
Negative control:
Not undertaken.
Sterility:
7 days plated sterility test.
Sabouraud Dextrose Broth is especially selective
for yeast and mold due to the low pH conditions.
Formulation:
Per liter of water adjusted
to pH 5.6 ± 0.2
Peptone
Dextrose
For agar add:
Agar
10.0 g
20.0 g
15.0 g
Organisms
Characteristics Coloring
C. albicans ATCC 10231 Growth Off-white,
creamy
E. coli ATCC 25922 Growth Off-white,
creamy
S. cerevisiae ATCC 9763 Growth Off-white
Historical background:
Sabouraud Dextrose Broth is a modification of dextrose agar described by
Sabouraud in 1892 for identification of fungi based on their cultural
characteristics. The medium depended solely upon its acid pH for
suppression of bacteria. Sabouraud Dextrose Agar, Emmons is a
modification with a neutral pH and reduced dextrose concentration.
Historically, the inhibitory action of Sabouraud Dextrose Agar on
microorganisms other than fungi was enhanced by the addition of agents
such as tellurite, copper sulfate, bile salts, dyes and antibiotics.
Sabouraud Dextrose Broth (Fluid Sabouraud Medium) is the liquid
counterpart of the agar prepared according to the formulation specified in
the U.S. Pharmacopoeia and National Formulary for sterility testing of
pharmaceutical products. Used in the quantitative identification of yeast
and mold.
Order information Sabouraud Dextrose Media
Product Description Qty/Pkg Order No
Ampouled Media 2 ml 50 10 496 157
Bottled broth 50 ml 8 10 496 702*
100 ml 1 10 496 785*
500 ml 1 10 496 786*
Bottled agar 50 ml 8 10 496 781*
100 ml 1 10 496 782*
500 ml 1 10 496 783*
Petri dishes with sterile pads 47 mm 100 10 498 544
Petri dishes with sterile pads 50 mm 50 10 445 905
* available on request
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