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Media<br />
Wallerstein Nutrient <strong>Broth</strong> (WL) and WL Differential <strong>Broth</strong> (WLD)<br />
Products<br />
Historical background:<br />
WL Nutrient Agar and WL Differential Agar were developed by GRAY and<br />
GREEN (1950), to study the microbial population of worts, beers, liquids,<br />
yeast, and other materials used in fermentation control procedures of the<br />
brewing industry and other fermentation industries.<br />
The WL Nutrient Agar is used for the cultivation and enumeration of yeast.<br />
At pH 5.5 brewers’ yeast will grow unrestricted at 25º C within 24–48 hours.<br />
For growth of bakers’ and distillers’ yeast, the pH of the medium should be<br />
adjusted to 6.5 with incubation at 30º C for 2–7 days. Where yeast cells are<br />
in low number, certain bacteria can be detected.<br />
The WL Differential <strong>Broth</strong> is used to determine bacterial counts. GRAY and<br />
GREEN (1950) recommended using the differential medium to culture<br />
bacteria normally encountered in brewing; aerobic conditions for acetic<br />
acid and thermophilic bacteria, anaerobic conditions for lactic bacteria and<br />
beer cocci.<br />
Wallerstein Differential <strong>Broth</strong> (WLD): Cultur of<br />
Lactobacillus plantarum ATCC 8014.<br />
Order information WL Nutrient Media<br />
Product Description Qty/Pkg Order No<br />
Ampoules 2 ml 50 10 496 108<br />
Petri dishes with sterile pads 47 mm 100 10 498 544<br />
Petri dishes with sterile pads 50 mm 50 10 445 905<br />
* available on request<br />
Order information WL Differential Media<br />
Product Description Qty/Pkg Order No<br />
Ampoules 2 ml 50 10 496 109<br />
Bottled Media 50 ml 8 10 496 717*<br />
Petri dishes with sterile pads 47 mm 100 10 498 544<br />
Petri dishes with sterile pads 50 mm 50 10 445 905<br />
* available on request<br />
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