Cetrimide Broth

Media
Trypticase Soy Broth – Double Strength
Products
Trypticase Soy Broth – Double Strength
General-purpose medium used in
qualitative procedures for the cultivation
of fastidious and non-fastidious
microorganisms. This media is also
recommended for sterility test
according to U.S. Pharmacopeia.
Description:
TSB broth is a medium that will support
the growth of a wide variety of
microorganisms including aerobic,
facultative and anaerobic bacteria and
fungi.
Interpretation:
When used as a broth adsorbed onto
pads TSB will support the development
of mixed cultures of bacteria and fungi.
When used in sterility test applications
turbidity indicates the presence of
organisms when compared to an
uninoculated control.
Quality Control and recommended
incubation conditions:
Positive controls:
Escherichia coli ATCC 25922,
incubated 24–48 hours at 35º C.
Negative control:
Not undertaken.
Sterility:
7 days plated sterility test.
Trypticase Soy Broth (Double Strength). Not
inoculated.
Formulation:
Per liter of water adjusted
to pH 7.3 ± 0.2
Pancreatic digest of casein
Papaic digest of soybean meal
Sodium chloride
Dipotassium phosphate
Dextrose
34.0 g
6.0 g
10.0 g
5.0 g
5.0 g
Organisms
Characteristics
B. subtilis ATCC 6633 Growth
C. albicans ATCC 10231 Growth
E. coli ATCC 25922 Growth
S. aureus ATCC 25923 Growth
Trypticase Soy Broth (Double Strength): left vial:
control, right vial inoculated with Escherichia coli
ATCC 25922.
Order information Trypticase Soy Broth (Double Strength)
Product Description Qty/Pkg Order No
Bottled broth 50 ml, W/M bottle 1 10 496 725
100 ml 1 10 496 708
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