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Media<br />
Trypticase Soy <strong>Broth</strong> (TSB)<br />
Products<br />
Trypticase Soy <strong>Broth</strong> (TSB)<br />
Trypticase Soy <strong>Broth</strong> (TSB) is a<br />
general-purpose medium used for<br />
the cultivation of most bacteria.<br />
Description:<br />
TSB broth is a medium that will support<br />
the growth of a wide variety of microorganisms<br />
including aerobic, facultative<br />
and anaerobic bacteria and fungi.<br />
Interpretation:<br />
When used as a broth adsorbed onto<br />
pads TSB will support the development<br />
of mixed cultures of bacteria and fungi.<br />
When used in sterility test applications<br />
turbidity indicates the presence of<br />
organisms when compared to an<br />
uninoculated control.<br />
Quality Control and recommended<br />
incubation conditions:<br />
Positive controls:<br />
Escherichia coli ATCC 25922,<br />
incubation for 24–48 hours at 35º C.<br />
Negative control:<br />
Not undertaken.<br />
Sterility:<br />
7 days plated sterility test.<br />
Ready to use Tryptic Soy <strong>Broth</strong> (USP). Not<br />
inoculated.<br />
Formulation:<br />
Per liter of water adjusted<br />
to pH 7.3 ± 0.2<br />
Pancreatic digest of casein<br />
Papaic digest of soybean meal<br />
Sodium chloride<br />
Dipotassium phosphate<br />
Dextrose<br />
17.0 g<br />
3.0 g<br />
5.0 g<br />
2.5 g<br />
2.5 g<br />
Organisms<br />
Characteristics<br />
B. subtilis ATCC 6633 Growth<br />
C. albicans ATCC 10231 Growth<br />
E. coli ATCC 25922 Growth<br />
S. aureus ATCC 25923 Growth<br />
Order information Trypticase Soy Media (TSB)<br />
Product Description Qty/Pkg Order No<br />
Ampoules 2 ml 50 10 496 160<br />
Bottled media 50 ml 8 10 496 791*<br />
100 ml 1 10 496 792*<br />
100 ml, Septa Cap 1 10 496 750*<br />
100 ml 1 10 496 707*<br />
Bottled agar 23 ml 15 10 496 862*<br />
Petri dishes with sterile pads 47 mm 100 10 498 544<br />
Petri dishes with sterile pads 50 mm 50 10 445 905<br />
* available on request<br />
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