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Media<br />

Trypticase Soy <strong>Broth</strong> (TSB)<br />

Products<br />

Trypticase Soy <strong>Broth</strong> (TSB)<br />

Trypticase Soy <strong>Broth</strong> (TSB) is a<br />

general-purpose medium used for<br />

the cultivation of most bacteria.<br />

Description:<br />

TSB broth is a medium that will support<br />

the growth of a wide variety of microorganisms<br />

including aerobic, facultative<br />

and anaerobic bacteria and fungi.<br />

Interpretation:<br />

When used as a broth adsorbed onto<br />

pads TSB will support the development<br />

of mixed cultures of bacteria and fungi.<br />

When used in sterility test applications<br />

turbidity indicates the presence of<br />

organisms when compared to an<br />

uninoculated control.<br />

Quality Control and recommended<br />

incubation conditions:<br />

Positive controls:<br />

Escherichia coli ATCC 25922,<br />

incubation for 24–48 hours at 35º C.<br />

Negative control:<br />

Not undertaken.<br />

Sterility:<br />

7 days plated sterility test.<br />

Ready to use Tryptic Soy <strong>Broth</strong> (USP). Not<br />

inoculated.<br />

Formulation:<br />

Per liter of water adjusted<br />

to pH 7.3 ± 0.2<br />

Pancreatic digest of casein<br />

Papaic digest of soybean meal<br />

Sodium chloride<br />

Dipotassium phosphate<br />

Dextrose<br />

17.0 g<br />

3.0 g<br />

5.0 g<br />

2.5 g<br />

2.5 g<br />

Organisms<br />

Characteristics<br />

B. subtilis ATCC 6633 Growth<br />

C. albicans ATCC 10231 Growth<br />

E. coli ATCC 25922 Growth<br />

S. aureus ATCC 25923 Growth<br />

Order information Trypticase Soy Media (TSB)<br />

Product Description Qty/Pkg Order No<br />

Ampoules 2 ml 50 10 496 160<br />

Bottled media 50 ml 8 10 496 791*<br />

100 ml 1 10 496 792*<br />

100 ml, Septa Cap 1 10 496 750*<br />

100 ml 1 10 496 707*<br />

Bottled agar 23 ml 15 10 496 862*<br />

Petri dishes with sterile pads 47 mm 100 10 498 544<br />

Petri dishes with sterile pads 50 mm 50 10 445 905<br />

* available on request<br />

66

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