Cetrimide Broth

Media
M-FC with Rosolic Acid
Products
M-FC with Rosolic Acid
M-FC Broth with Rosolic Acid is used
for the detection of fecal coliforms by
the membrane filtration technique.
Description:
M-FC with Rosolic Acid acts and
functions in the same way as m-FC
Broth. Rosolic acid inhibits bacterial
growth in general, except for fecal
coliforms.
Interpretation:
Bile salts inhibit non-enteric bacteria.
Aniline blue indicates the ability of fecal
coliforms to ferment lactose to acid
that causes a pH change in the
medium. Other organisms form grey to
cream colonies.
Organisms
Characteristics Coloring
E. coli ATCC 25922 Growth Blue
E. aerogenes ATCC 13048 Growth Gray to
cream
E. faecalis ATCC 29212 inhibited
Quality Control and recommended
incubation conditions:
Positive control:
Escherichia coli ATCC 25922,
24 hours at 44,5º C.
Negative control:
Enterobacter aerogenes ATCC 13048,
24 hours at 44.5º C.
Sterility:
7 days plated sterility test.
M-FC with Rosolic Acid: Escherichia coli ATCC
25922 forms blue colonies whereas non-lactose
fermenters appears as grey colonies.
Formulation:
Per liter of water adjusted to pH 7.4
Tryptose
Peptone No. 3
Yeast extract
Sodium chloride
Lactose
Bile salts
Aniline blue
Rosolic Acid 1%
10.0 g
5.0 g
3.0 g
5.0 g
12.5 g
1.5 g
0.1 g
10.0 ml
Additional information:
Fecal coliform (FC) Medium for the membrane filtration technique was
described by Geldereich et al. in 1965. It was the first membrane filtration
technique to be incubated at 44.5 ± 0.2.
Order information m-FC with Rosolic Acid
Product Description Qty/Pkg Order No
Ampouled Media 2 ml 50 10 496 114
Bottled broth 50 ml 8 10 496 719*
Petri dishes with sterile pads 47 mm 100 10 498 544
Petri dishes with sterile pads 50 mm 50 10 445 905
* available on request
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