CAGE PROCESSING in Animal Facilities - Felasa

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7.3.1 WashingThe washing performance is assessed according to DIN 10511. The water bottles are contaminated directlywith test soiling. No extra test strips are needed. The relevant test number of items as well as the number ofthe wash cycles are indicated in table 7-2. The assessment is done on a visual basis.7.3.1.1 Producing the test soilingThe test soiling for contamination is made of reconstituted skimmed milk. For producing 100 ml of reconstitutedskimmed milk, 10 g skimmed milk powder is mixed with 100 ml distilled water. The mixture must be stirredvigorously before being steam sterilised for 5 minutes at 121 °C. For extra good adhesion, 13 g celluloseshould be added before sterilising.Table 7-3Chemicalspray-dried skimmed milk powdercellulose (microcrystalline for thin-layer chromatography)Source of supplypharmacyFLUKA chemicals catalogue, CH-Buchs7.3.1.2 Applying the test soilingA water bottle should be half-filled with test soiling and be emptied by giving it a rotation in a tilted position, sothe entire inside of the bottle is wet with test soiling. For wetting the bottle rim, it is recommended to dip the rim1 cm deep into the test soiling. The drying process takes 2 hours altogether with the bottles being first placedupside down in a bottle crate and turned over after an hour.The assessment is done visually and differentiates between clean and soiled bottles (please refer to figuresbelow). Acceptance criteria: All test bottles must be visually clean.Figure 7-7 Contaminated test bottle Figure 7-8 Still soiled test bottle Figure 7-9 Clean test bottle7.3.2 DecontaminationWater bottles do not get soiled and contaminated as much as cages and other items for processing do (e.g.with excrement, urea, etc.). Therefore, the decontamination of water bottles is not equally relevant. Although itis generally possible to check water bottles for decontamination, it needs a very complex procedure. The reasonis, that there are no standardisable test strips, and each bottle type would have to undergo an individualprocedure. It is therefore recommended by the working group to sterilise water bottles whenever a hygienicstandardisation of the processing procedure for water bottles is required. This approach is also common practice.54Cage Processing in Animal Facilities · 4th issue · 2013
7.4 Test procedure for confirming the rinsing of cage bases, wire lids, racks,water bottles, and bottle capsWhen using alkaline and acid detergents, the rinsing effectiveness can be tested by means of pH indicators.The effectiveness is tested on the items after the rinsing procedure is finished.Table 7-4Detergent pH Indicator pH ChangerangeImplementationColour reactionalkalinephenolphthaleinsolution*9,4 - 10,6 Residual moisture is soaked upwith a cloth. For determining the pHvalue, the cloth is wetted with a fewdrops of indicator solution.reddish purpleindicates alkalinityalkalinephenolphthaleinpaper9,4 - 10,6 Following the manufacturer instructions,the paper is dipped in orwetted with adherent residual moisture.The colour value can then beread off the paper.reddish purpleindicates alkalinityacid methyl orange** 3,0 - 4,4 Residual moisture is soaked upwith a cloth. For determining the pHvalue, the cloth is wetted with a fewdrops of indicator solution.Red indicatesacid; else theindicator solutionwould be yellow-orange.alkalineand acidlitmus paper / pHindicator sticksall pH rangesFollowing the manufacturer instructions,the paper is dipped in orwetted with adherent residual moisture.The colour value can then beread off the paper.according to manufacturerinstructions*1% in ethanol / **highly diluted solution (0,04g methyl orange per 100ml of 20% ethanol)Please note: Due to its alkalinity, softened rinsing water can misleadingly indicate residues of alkaline detergents.(Demineralised water that is often used for rinsing can have an “acid” pH value and misleadingly indicateresidues of acid detergents.)7.5 Test procedures for drying of cage basesThe drying result is assessed visually. The levels of assessment are depicted below:Figure 7-10 Cage body, without any visible waterresidues or drops leftFigure 7-11 Cage body, only some drops visibleCage Processing in Animal Facilities · 4th issue · 2013 55
Page 1 and 2:
CAGE PROCESSINGin Animal Facilities
Page 3 and 4:
CAGE PROCESSINGin Animal Facilities
Page 5 and 6: Index1 Introduction ...............
Page 7 and 8: 7.1 Requirements ..................
Page 9 and 10: 2 Cage processing cyclesThe followi
Page 11 and 12: 3.2 Materials for processingWhen de
Page 13 and 14: • They should be easy and conveni
Page 15 and 16: • Height-adjustable shelves ensur
Page 17 and 18: Table 4-2Option Advantages Disadvan
Page 19 and 20: Below you will find a description o
Page 21 and 22: Figure 4-10 Tunnel cage washer4.3.1
Page 23 and 24: WashingHot water mixed with deterge
Page 25 and 26: Loading systemsIn cabinet cage wash
Page 27 and 28: 4.3.2.1.3 Rinse aidRinse aids shoul
Page 29 and 30: Semiautomaticdispensing*bedding is
Page 31 and 32: Semi-automaticsystemsmultiple filli
Page 33 and 34: PermissionsWith frequent changes in
Page 35 and 36: 4.6.2.1 Basic lock types for H 2O 2
Page 37 and 38: configurations can thus require ind
Page 39 and 40: 4.6.3.2 Processual requirementsPAA
Page 41 and 42: Lead mg/l -heavy metal residues (ex
Page 43 and 44: For reasons of odour, exhaust air o
Page 45 and 46: 5.6 Installation data and consumpti
Page 47 and 48: 6.2 Start up of operationIt is part
Page 49 and 50: 6.9 Routine checks and measuresThe
Page 51 and 52: 7.2.1.1.2 Test setup1 inside at the
Page 53 and 54: 7.2.2 Test procedures for evaluatin
Page 55: surfaces stay soil free. For an eve
Page 59 and 60: Table 7-6WasherItems for processing
Page 61 and 62: with rinse aid, for example, can be
Page 63 and 64: 9 Potential defects and damages in
Page 65 and 66: Table 9-3Source and causesStress /
Page 67 and 68: Table 9-6Sources and causesWashing
Page 69 and 70: DIN 1986:Technical Rules for Drinki
Page 71 and 72: Detergent liquor / detergent soluti
Page 73 and 74: ProcessingProcessing means washing,
Page 75 and 76: Final provisions:1. This brochure d
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CAGE PROCESSING in Animal Facilities - Felasa

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