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+ R - Center for Nanoscale Systems - Harvard University

+ R - Center for Nanoscale Systems - Harvard University

+ R - Center for Nanoscale Systems - Harvard University

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Optical MicroscopyCapabilities– More than 10 OMs at CNS: Stereo, Reflection,Transmission, Inverted, Fluorescence….– Modes: BF, DF, Differential Interference Contrast(DIC), Polarizing– Objective Lenses: 2.5x to 150x– Ultra-High Definition Digital Cameras (6.7um/pixel)-Advance Optical Techniques: NSOM, Confocal, TIRF,TRPL, Raman,…(Contact Jiangdong Deng or Arthur McClelland)Advantages– Rapid– Non-destructive– Lager Area InspectionDisadvantages– Lateral Resolution is Limited by Rayleigh Criterion– Poor Vertical Resolution and No Height (Z)In<strong>for</strong>mation100xαR = 0.61λ/ NAN.A = n*sin α0.2 mm WDNA ~ 0.8-0.9520x

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