EKSpot MALDI Spotter System Operator Guide - Eksigent

Operate the EKSpot MALDI Spotter SystemSet the PreFlush OptionThe preflush option instructs the system to flush the matrix addition capillary at a higher flow ratebefore each run1. In the Configuration tab in the ekspot Configuration dialog (Figure 5-2), select theEnabled check box to enable the preflush option.Figure 5-2ekspot Configuration dialog—Configuration tab2. Type a value (in µL/min) in the Flowrate field for the preflush flow rate.A flow rate of 20 µL/min is recommended.Flow rates higher than 20 µL/min may generate high back pressure and can causeleakage in the syringe pump valve.3. Type a value (in minutes) in the Duration field for the preflush duration.Be sure to set the duration long enough to completely flush the matrix additioncapillary.EKSpot MALDI Spotter SystemOperator Guide24 of 38 September 2012