EKSpot MALDI Spotter System Operator Guide - Eksigent

Set up the Run Manager WindowTable 6-1ColumnAutosampler MethodAutosampler TrayAutosampler VialLC MethodLC ChannelLC Flowrate (nL/min)Spotter Plate #Spotter Start RowSpotter Time/Drop (s)Spotter Time (min)Columns Available in the Run TableSpotter Matrix Flow (µL/min)Other StatusFunctionSelects the autosampler method to be used in the run.Double-click the cell to activate the menu, and then select theautosampler method.Selects the autosampler tray where the sample vial is placed.Default tray is 1.Selects the sample vial used in the run.Double-click the cell to activate the menu, and then select thevial. As an alternative, click the vial holder picture to select thevial.Selects the LC method to be used in the run.Double-click the cell to activate the menu, and then select theLC method.Selects the LC channel where the method is going to be run.Channel 1 is the high flow channel and Channel 2 is the lowflow channel.Double-click the cell to activate the menu, and then select theLC Channel.Sets the LC flow rate.If this cell is blank, the flow rate defined in the LC method isused.Selects the target plate for spotting.Double-click the cell to activate the menu, and then select theplate number. Refer to Figures 5-4 and 5-5 for plate numberassignment.Selects the row where the spotting is to start.Sets the size/duration of the spot in msec.Sets the time interval in the LC method for spotting.It is possible to have multiple intervals in the same LC run. Forexample, if 0-5 min and 10-23 min are entered in the column,the spotter will spot from 0 min to 5 min and from 10 min to23 min in the LC method.Sets the matrix flow rate in µL/min.Shows the status of the run.Operator GuideEKSpot MALDI Spotter SystemSeptember 2012 33 of 38