Muse™ Cell Analyzer

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About this GuideThe Muse Cell Analyzer User’s Guide provides detailed information onoperating and maintaining the Muse System. This guide providesinstructions for setting up the system, managing users and data files,running a System Check procedure, and cleaning the system. It does notinclude instructions for using the software modules to acquire samples andanalyze data.For information on preparing samples and running them on the instrument,refer to the specific kit user’s guide located at www.millipore.com/muse.Conventions Used in This Guide■ NOTE: Points out additional information that may be helpful.▲ WARNING: Alerts you to situations that could result in bodily harm,instrument damage, failure in a procedure, or incorrect results.Help1 Read through the section of the guide specific to the operation you areperforming. Refer to the table of contents and index to locateinformation. A glossary is included to assist you with any unfamiliarterms.2 See the troubleshooting section for a list of problems and suggestedsolutions.3 Refer to the technical support contact information below:• For ordering information or technical support, call toll-free in theUSA and Canada:Phone: +1 (800) MILLIPORE (645-5476)Fax: +1 (800) 645-4539• Outside the US, visit www.millipore.com/offices for up-to-dateworldwide contact information.4 For information on performing an assay, refer to the specific kit user’sguide, which can be found at www.millipore.com/muse.2 Muse Cell Analyzer User’s Guide
SafetyThe Muse System is equipped with safety features for your protection. Usethe system only as directed in this user’s guide. Do not perform instrumentmaintenance or service except as specifically stated. Read the followingsafety information before using the system.General Safety▲ WARNING: If this instrument is not used in the manner indicated bythe instructions in this guide, the safety features of the instrument maybe impaired. Follow these guidelines:• Use only the tubes specified. The use of tubes other than thosespecified may result in damage to the instrument.Biological Safety▲ WARNING: All biological specimens and materials that come intocontact with them can transmit potentially fatal disease. To preventexposure to biohazardous agents, follow these guidelines:• Handle all biological specimens and materials as if capable oftransmitting infection. Dispose of waste using proper precautionsand in accordance with local regulation. Never pipette by mouth.Wear suitable protective clothing, eyewear, and gloves.• After emptying waste bottle, add bleach to the first (fill) line. Ittakes approximately 10 mL of bleach to reach the fill line. Disposeof waste in accordance with federal, state, and local regulations.Electrical Safety▲ WARNING: Power off the instrument using the Power Options tool onthe main menu and disconnect the power cord before replacing fuses.Laser SafetyThe Muse System contains a Class IIIb laser operating at 532 nm in CWmode. Light shields within the instrument enclose the path of laserradiation. Additionally, the instrument enclosure provides secondaryprotection from any laser radiation.Preface 3
Page 1: Muse Cell AnalyzerUser’s GuideFor
Page 6 and 7: viMuse Cell Analyzer User’s Guide
Page 10 and 11: ▲ WARNING: To avoid exposure to l
Page 12 and 13: Setting Up Your SystemYour Muse Sys
Page 14 and 15: 8 Muse Cell Analyzer User’s Guide
Page 16 and 17: Sample LoaderThe sample loader hold
Page 18 and 19: Software OverviewUse the software t
Page 20 and 21: Favorite Assay ButtonsThe main menu
Page 22 and 23: FilesFor each assay, the software s
Page 24 and 25: WarningsSystem Check:• Loader arm
Page 26 and 27: File StorageThe Muse System has an
Page 28 and 29: Retrieving Instrument Settings1 Fro
Page 30 and 31: Assay OverviewDetailed steps for ea
Page 32 and 33: About DialogThe About dialog provid
Page 34 and 35: 3 Fill the cleaning solution bottle
Page 36 and 37: sets as a default Administrator, as
Page 38 and 39: Logging On Once Users are AddedWhen
Page 40 and 41: 1 If you forgot your PIN, select Do
Page 42 and 43: Selecting an Assay to RunThe main m
Page 44 and 45: 4 Select Run System Check.Run Syste
Page 46 and 47: advancing, the fluid system may be
Page 48 and 49: System Check ResultsThe result appe
Page 50 and 51: Viewing Past System Check ResultsTo
Page 52 and 53: Exporting the Check LogThe System C
Page 54 and 55: Legend ItemLimit LowTargetLimit Hig
Page 56 and 57: 4 Select the USB drive from the Exp
Page 58 and 59:
• Reset Fluid Levels sets the flu
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• 10% bleach solution in Guava IC
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2 Select Run Complete Clean.Display
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5 If you are shutting down the syst
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select View Log/Results from the Co
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2 You can filter the list to view s
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Filling the Cleaning Solution Bottl
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7 Replace the cap and reinstall the
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3 Use your fingers to unscrew the t
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12 Place the fluidics box (with flo
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3 Select Eject USB in the title bar
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To Access the Users & Access Levels
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Editing User InformationAny user ca
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■NOTE: Only administrators can ed
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The Edit Favorites screen now appea
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Exporting by DateYou can export dat
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e overwritten. Select YES to procee
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5 Select YES to install the softwar
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ProblemSystem Check failures: oneor
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90 Muse Cell Analyzer User’s Guid
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flow cellAn optical assembly within
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Fluidicsflow cell dimension:pump:sa
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WarrantyEMD Millipore Corporation (
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