Rice Genetics IV - IRRI books - International Rice Research Institute

First inriceHexa3%Mono4%Penta12%Di26%Tri45%Tetra10%1.2% inrice ESTsDistribution of simple sequence repeats in 1,260 STCs. A total of 56,715 STCs were examinedby Repeat Masker program (mono = 54, di = 333, tri = 555, tetra = 124, penta =153, and hexa = 41).Fig. 3. Frequency and distribution of simple sequence repeats in the HindIII sequence-taggedconnector database. ESTs = expressed sequence tags.Table 3. CCW chromosome 3 and 10 sequencing timetable.ActivityYear 1 Year 2 Year 3Sep 1999-Aug 2000 Sep 2000-Aug 2001 Sep 2001-Aug 2002Clone validation 40 BACs 62 BACs 58 BACsProduction sequencing 40 BACs 62 BACs 58 BACs5.2 Mb draft 8.06 Mb draft 7.54 Mb draftFinishing/annotation (I) 32 BACs 48 BACs 80 BACsFinished data release 4.16 Mb 6.24 Mb 10.40 MbThe CCW Rice Genome Sequencing ConsortiumRice genome sequencing at CCW is divided into three sites. CUGI, led by R. Wing, isresponsible for overall management of the project, physical mapping, clone selection/validation,production sequencing, finishing, and annotation. CSHL, led by W.R.McCombie, is responsible for production sequencing, finishing and finishing management,and annotation. Scientists at WU-GSC, led by R. Wilson, are responsiblefor finishing difficult clones and developing novel strategies and chemistries to finishdifficult clones. Table 3 outlines our goals for finishing the short arms of chromosomes10 and 3 over the 3-year project.The short arm of chromosome 10—difficult to finishUsing the CUGI Rice Genome Framework and all other available resources (primarilyfrom the Japanese Rice Genome Program and Cornell University: genetic mark-220 Wing et al