Rice Genetics IV - IRRI books - International Rice Research Institute

1999, Krysan et al 1999, Sato et al 1999). The identification of the desired mutantcould be accomplished efficiently using a super-pooling strategy as suggested byKrysan et al (1999). They estimated that the maximum useful pool size is 2,350 linesin Arabidopsis based on the sensitivity for detecting a specific T-DNA insert and thetotal amount of template DNA. We are performing experiments to determine the uppersize limit on DNA pools of the rice tagged lines.Our T-DNA tagging lines will be useful in analyzing the function of several valuablegenes by various approaches. With the increasing availability of rice genomesequences from public databases, it would be valuable to construct a database for theflanking sequences. The development of effective methods for amplification of sequencesadjoining the insertion would enable us to construct the database effectively.ReferencesAzpiroz-Leehan R, Feldmann KA. 1997. T-DNA insertion mutagenesis in Arabidopsis: goingback and forth. Trends Genet. 13:152-156.Babiychuk E, Fuangthong M, Van Montagu M, Inze D, Kushnir S. 1997. Efficient gene taggingin Arabidopsis thaliana using a gene trap approach. Proc. Natl. Acad. Sci. USA 94:12722-12727.Campisi L, Yang Y, Yi Y, Heilig E, Herman B, Cassista AJ, Allen DW, Xiang H, Jack T. 1999.Generation enhancer trap lines in Arabidopsis and characterization of expression patternsin the inflorescence. Plant J. 17:699-707.Cooley MB, Goldsbrough AP, Still DW, Yoder JI. 1996. Site-selected insertional mutagenesisof tomato with maize Ac and Ds elements. Mol. Gen. Genet. 252:184-194.Couteau F, Belzile F, Horlow C, Grandjean O, Vezon D, Doutriaux MP. 1999. Random chromosomesegregation without meiotic arrest in both male and female meiocytes of a dmc1mutant of Arabidopsis. Plant Cell 11:1623-1634.Feldmann KA. 1991. T-DNA insertion mutagenesis in Arabidopsis: mutational spectrum. PlantJ. 1:71-82.Frey M, Stettner C, Gierl A. 1998. A general method for gene isolation in tagging approaches:amplification of insertion mutagenized sites (AIMS). Plant J. 13:717-721.Hiei Y, Komari T, Kubo T. 1997. Transformation of rice mediated by Agrobacterium tumefaciens.Plant Mol. Biol. 35:205-218.Hiei Y, Ohta S, Komari T, Kumashiro T. 1994. Efficient transformation of rice (Oryza sativaL.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA.Plant J. 6:271-282.Ishida Y, Saito H, Ohta S, Hiei Y, Komari T, Kumashiro T. 1996. High efficiency transformationof maize (Zea mays L.) mediated by Agrobacterium tumefaciens. Nat. Biotechnol.14:745-750.Jefferson RA, Kavanagh TA, Bevan MW. 1987. GUS fusions: β-glucuronidase as a sensitiveand versatile gene fusion marker in higher plants. EMBO J. 6:3901-3907.Kertbundit S, De Greve H, Deboeck F, Van Montagu M, Hernalsteens JP. 1991. In vivo randomβ-glucuronidase gene fusions in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 88:5212-5216.Kertbundit S, Linacero R, Rouze P, Galis I, Macas J, Deboeck F, Renckens S, Hernalsteens JP,De Greve H. 1998. Analysis of T-DNA-mediated translational β-glucuronidase gene fusions.Plant Mol. Biol. 36:205-217.Generation of T-DNA insertional tagging lines in rice 261