Rice Genetics IV - IRRI books - International Rice Research Institute

ate in 2% O 2 can be limited by Pi regeneration capacity (Sharkey 1985), high-levelexpression of PEPC might affect Pi regeneration capacity in transgenic rice.Transgenic rice plants overexpressing the maize PPDK showed a higher photosyntheticrate (up to 35%) than untransformed plants. This increased photosyntheticcapacity is at least in part due to an enhanced stomatal conductance and a higherinternal CO 2 concentration. We are now crossing these transgenic rice plants to integrateboth PEPC and PPDK genes into the same transgenic rice plants.ReferencesFurbank RT, Taylor WC. 1995. Regulation of photosynthesis in C 3 and C 4 plants: a molecularapproach. Plant Cell 7:797-807.Gehlen J, Panstruga R, Smets H, Merkelbach S, Kleines M, Porsch P, Fladung M, Becker I,Rademacher T, Hausler RE, Hirsch HJ. 1996. Effects of altered phosphoenolpyruvate carboxylaseactivities on transgenic C 3 plant Solanum tuberosum. Plant Mol. Biol. 32:831-848.Glackin CA, Grula JW. 1990. Organ-specific transcripts of different size and abundance derivefrom the same pyruvate, orthophosphate dikinase gene in maize. Proc. Natl. Acad. Sci.USA 87:3004-3008.Goodall GJ, Filipowicz W. 1991. Different effects of intron nucleotide composition and secondarystructure on pre-mRNA splicing in monocot and dicot plants. EMBO J. 10:2635-2644.Hudspeth RL, Grula JW, Dai Z, Edwards GE, Ku MSB. 1992. Expression of maize phosphoenolpyruvatecarboxylase in transgenic tobacco. Plant Physiol. 98:458-464.Imaizumi N, Ku MSB, Ishihara K, Samejima M, Kaneko S, Matsuoka M. 1997. Characterizationof the gene for pyruvate, orthophosphate dikinase from rice, a C 3 plant, and a comparisonof structure and expression between C 3 and C 4 genes for this protein. Plant Mol. Biol.34:701-716.Kogami H, Shono M, Koike T, Yanagisawa S, Izui K, Sentoku N, Tanifuji S, Uchimiya H, TokiS. 1994. Molecular and physiological evaluation of transgenic tobacco plants expressing amaize phosphoenolpyruvate carboxylase gene under the control of the cauliflower mosaicvirus 35S promoter. Transgenic Res. 3:287-296.Lipka V, Häusler RE, Rademacher T, Li J, Hirsch H-J et al. 1999. Solanum tuberosum doubletransgenic expressing phosphoenolpyruvate carboxylase and NADP-malic enzyme displayreduced electron requirement for CO 2 fixation. Plant Sci. 144:93-105.Matsuoka M, Tada Y, Fujimura T, Kano-Murakami Y. 1993. Tissue-specific light-regulatedexpression directed by the promoter of a C 4 gene, maize pyruvate, orthophosphate dikinase,in a C 3 plant, rice. Proc. Natl. Acad. Sci. USA 90:9586-9590.Matsuoka M, Kyozuka J, Shimamoto K, Kano-Murakami Y. 1994. The promoters of two carboxylasesin a C 4 plant (maize) direct cell-specific light-regulated expression in a C 3 plant(rice). Plant J. 6:311-319.Matsuoka M, Fukayama H, Tsuchida H, Nomura M, Agarie S, Ku MSB, Miyao M. 2000. Howto express some C 4 photosynthesis genes at high levels in rice. In: Sheehy JE, Mitchell PL,Hardy B, editors. Redesigning rice photosynthesis to increase yield. Proceedings of theWorkshop on The Quest to Reduce Hunger: Redesigning Rice Photosynthesis, 30 Nov.-3Dec. 1999, Los Baños, Philippines. Makati City (Philippines): International Rice ResearchInstitute and Amsterdam (The Netherlands): Elsevier Science B.V. p 167-175.446 Matsuoka et al