Rice Genetics IV - IRRI books - International Rice Research Institute

Table 4. CCW sequencing status for year 1.Goal CUGI CSHL WUGSC CCW Sequence (Mb)Finishing 12 20 0 32 4.17Finished 3 5 1 9 1.39In finishing 12 16 4 32 4.72In production 0 0 0 0 0.00In library 0 0 0 0 0.00Total 15 21 5 41 6.11Sequence (Mb) 2.17 3.21 0.74 6.11immediate access to the rice genome for positional cloning and marker-assistedselection. Moreover, it would allow the IRGSP to provide a set of defined BAC clonesto sequence to each member of the IRGSP based on sequencing capacity and funding.Such a scheme would accelerate the completion of the sequencing of the rice genome,possibly as early as 2004.ReferencesArumuganathan K, Earle E. 1991. Nuclear DNA content of some important plant species. PlantMol. Biol. Rep. 9:208-219.Budiman M. 1999. Chapter III: Construction and characterization of a BAC library of Oryzasativa L. subsp. japonica cv. Nipponbare for genomic studies. PhD dissertation. TexasA&M University, College Station, Texas, USA. p 50-80.Cheng Z, Presting G, Buell C, Wing R, Jiang J. 2001. High resolution pachytene chromosomemapping of bacterial artificial chromosomes anchored by genetic markers reveals the centromerelocation and the distribution of genetic recombination along chromosome 10 ofrice. Genetics 157:1749-1757.Mao L, Wood T, Yu Y, Budiman M, Woo S, Sasinowski M, Goff S, Dean R, Wing R. 2000. Ricetransposable elements: a survey of 73,000 sequence-tagged-connectors (STCs). GenomeRes. 10:982-990.Marra M, Kucaba T, Dietrich N, Green E, Brownstein B, Wilson R, McDonald K, Hillier L,McPherson J, Waterston R. 1997. High throughput fingerprint analysis of large-insert clones.Genome Res. 7:1072-1084.Sasaki T, Burr B. 2000. International Rice Genome Sequencing Project: the effort to completelysequence the rice genome. Curr. Opin. Plant Biol. 3:138-141.Soderlund C, Humphray S, Dunham A, French L. 2000. Contigs built with fingerprints, markersand FPC V4.7. Genome Res. 10:1772-1787.Sulston J, Mallett F, Durbin R, Horsnell T. 1989. Image analysis of restriction enzyme fingerprintautoradiograms. CABIOS 13:101-106.Venter C, Smith H, Hood L. 1996. A new strategy for genome sequencing. Science 381:364-366.Yuan Q, Liang F, Hsiao J, Zismann V, Benito M, Quackenbush J, Wing R, Buell R. 2000.Anchoring of rice BAC clones to the rice genetic map in silico. Nucleic Acids Res. 28:3636-3641.224 Wing et al