Retriever IV Fraction Collector User Manual - Isco

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Retriever IV Fraction CollectorSection 3 Operating ProceduresTable 3-1 Retriever IV Connection CablesPart Number Connection Function68-1010-103 Pumps or other instrumentsShuts off pump or other instrument with 1 amp or less at endof run.68-1020-159 TRIS pump Volumetric collection and shut off.68-1020-161 TRIS pump Pump shut off at the end of the run.68-1020-213 Current UA-6 and V 4 detectorMarks tube changes on chart recorder and provides peakseparator interface.68-1020-083 Teledyne Isco detector Recorder stop at the end of the run.68-1010-111 Non-Teledyne Isco detector External control by non-Teledyne Isco detector.68-1020-188 Valve Connects two valves3.13 Connection to aComputer or otherExternal ControllerThe Retriever IV may be controlled externally via the 15-pinMONITOR connector. The monitor plug is shown in Figure 3-6numbered as seen from the rear of the Retriever IV. The matingconnector is a Cinch DA-15P Sub D or equivalent. Further informationcan be found in Table 5-2.Cable 68-1010-111 can be used for interfacing with Teledyne IscoRetriever IV fraction collectors. One end of this cable has a15-pin connector which connects to the MONITOR plug of theRetriever IV and the other end has 10 wires. The most commonapplication involves remote control of fraction changes, but thiscable also provides connections for sending tube change eventmarks to the recording instrument being used. The function andrelated wire connections are described in Table 3-2.The Retriever IV will advance one tube position upon receipt of acontact closure across pins 2 (+12 VDC) and 9 (circuit common).This signal must be 22 milliseconds or greater in duration toadvance tube positions.NotePin 2 has +12 VDC in reference to circuit common, therefore itis not TTL compatibleFigure 3-6 Monitor Plug Pin Designation3-10
Retriever IV Fraction CollectorSection 3 Operating ProceduresTable 3-2 Connector Pin DescriptionPin Wire Color Use1 Red +12 Volts2 Brown External tube change input5 Yellow Tube changing event mark6 Orange External pause8 Green Earth9 White Common11 Blue Motor Stop12 Black Valve13 Violet Pause count14 Grey End of run output3.14 Connection to TRISPumpsExample:3.15 Connection toNon-Teledyne IscoPumpsThe recommended cable for stopping the TRIS pump after theRetriever IV has completed its run is P/N 68-1020-161.An alternative cable connection to allow the fractions to be collectedvolumetrically from the TRIS pump is P/N 68-1020-159.With this connection, the TRIS pump sends electronic pulsesbased upon volume displaced to the fraction collector.The TRIS pump transmits at 267 pulses (open collector transistoroutput) per 10 revolutions of its rotor assembly. Thesepulses can be counted by Isco fraction collectors to allow collectionof fractions based upon fractions of pump revolutions. Setthe count switches to the desired volume to be pumped to eachfraction collector tube (1 ml is approximately 22 counts with 1/8"ID tubing; 78 counts with 1/16" ID tubing). Allow the pump tooperate at the selected speed for approximately 30 minutes towear in the tubing. Connect the pump to the fraction collector,measure the fluid pumped, then adjust the count in the fractioncollector to the desired accuracy. This step is necessary, since theID of standard silicone tubing can vary as much as 10%.Fractions of 5 ml are required and the pump is found to dispense1.21 ml per rotor revolutions, or 5 ml in 4.56 revolutions. At 267pulses per 10 rotor assembly revolutions, TRIS will send out 110pulses for each 5 ml pumped (22 pulses/ml with 1/8" tubing). Byprogramming the fraction collector to collect 110 units in thevolume mode, 5 ml fractions will be collected.When a non-Teledyne Isco pump is used, a cable is used tointerrupt mains power to the pump after the last tube has beenfilled. For pumps operating on 117 Vac at 1 amp or less, cable P/N68-1010-103 is used.3-11
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Retriever IV Fraction Collector User Manual - Isco

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