Retriever IV Fraction Collector User Manual - Isco

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Retriever IV Fraction CollectorSection 3 Operating Procedures3.16 Racks Three rack types are available for the Retriever IV (see Table 3-3and Figure 3-7). All racks have a stand, which can be rotated tosupport the rack when it is removed from the Retriever IV.Table 3-3 Available Rack Sets for the Retriever IVPart No. Tubes Accommodated Tubes per Rack Retriever IV Capacity68-0827-059 10 to 18 mm OD, 17 mm vials 10 190 tubes in 19 racks68-0827-060 25 mm OD 7 133 tubes in 19 racks68-0827-061 28 mm Scintillation vials 7 133 vials in 19 racks68-2187-00268-0827-05910 x 10 to 18 mm68-0827-0617 x 28 mm68-0827-0607 x 25 mmFigure 3-7 Available Tube Racks3.17 Multi-SourceCollection AdapterThe optional multi-source adapter (68-2267-001) is designed toallow collection of up to 19 fractions from as many as 10 chromatographiccolumns or other sources. 2.3.) The adapter (Figure3-8) serves as a tubing connection station for 7 inlets when 28mm scintillation vials are used, or for 10 inlets when 13, 16 or 18mm tubes are used. Special tubing connectors (P/N 60-2263-016)are supplied with the adapter to hold the tubing over the tubes orvials and serve as drop formers. These connectors use the sametubing ferrules as described in Figure 3-1.A rear panel switch (Figure 1-2) changes the operation of thefraction collector from stopping at each tube position in a rack tostopping only once per rack. The multi-source adapter may beinstalled over the rear or front racks of the collector. When thefraction collector advances to the next rack, the racks along therear of the collector move in one, approximately 0.8 second step.The advancement of the racks along the front of the collectoroccurs in two steps. The first requires approximately 0.7 secondsand accounts for approximately 80% of the total shiftingmovement and the remainder of the advancement occurs approx-3-12
Retriever IV Fraction CollectorSection 3 Operating Proceduresimately 4 seconds later. For this reason, the adapter should normallybe mounted over the rear racks of the fraction collector asshown in Figure 3-8. When using 16 mm or larger tubes, theadapter can be aligned over the front racks and adjusted so thatdrops falling from both advancement steps fall into the tubes.Using the front position is detailed in the paragraphs describingthe use of two multi-source adapters at the end of this section.The flow diverter on the adapter can be used to direct flow awayfrom the fraction collector at the end of a run. The diverter has aspring loaded trough which swings into place when it encountersa rubber stopper placed in the last rack in the run. As shown inFigure 3-8, the trough swings into place and diverts flow into awaste vessel placed beside the collector.The following procedure should be used to set up themulti-source adapter over the rear racks.1. If not already in place, install the right stainless steel columnsupport mast and tighten firmly in place.2. Slide the multi-source adapter over the mast and temporarilytighten it so it extends out the rear of the unit as picturedin Figure 3-8.3. Loosen the four small thumbscrews on the side of theadapter. Connect the tubing from the multiple sources toeach of the special tubing connectors supplied, using thetubing ferrules as described in Figure 3-1. Using the markingson the top of the multi-source adapter as a guide,install the seven or ten tubing connectors in their properpositions (for use with seven or ten tube racks, respectively)and then tighten the four small thumbscrews on theside of the adapter to hold the tubing connectors in place.4. Set the PAUSE/RUN switch in the PAUSE position. Usinga screwdriver, or other pointed tool, set the rear panelRACKS/ TUBES switch to the RACKS position.5. Depress and hold the ADVANCE switch until the fractioncollector advances to the red stop rack and then release theswitch allowing the unit to stop at the final position of thered stop rack.3-13
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