Retriever IV Fraction Collector User Manual - Isco

Retriever IV Fraction CollectorSection 5 Maintenance5.4 Shuttle Cleaning andRemovalIf the fraction collector shuttles mechanically jam, they haveprobably become clogged with foreign material. Remove the casetop and wash it in a stream of warm water and detergent whilegently shaking the individual shuttles. Rinse in warm water. Ifthis does not clear the jam, the shuttles will have to be removedfor cleaning, replacement of broken shuttles, or retiming ofshuttle adjustment.If it becomes necessary to remove the shuttles due to grossspillage or breakage, proceed with the following steps:1. Remove the case top (see section 5.2) and place on a flatsurface.2. Remove all the test tube racks.3. Remove the four screws on the center rail plate andremove.NoteDo not remove the two end shuttles from the narrow passagesat the ends of the center rail unless absolutely necessary. Ifthese shuttles are removed, refer to section 5.5 for propershuttle timing when the unit is reassembled.4. Remove the shuttles and inspect each shuttle for damageto gear rack, coupling, and camming surface.5. Discard and replace any damaged shuttles.6. Clean and reassemble the unit. Be sure all 19 shuttles arein place and oriented in the same direction.7. For general cleaning of the Retriever IV’s front panel orenclosure, use a mild detergent in water or isopropyl alcoholon a sponge that has been mostly squeezed out.8. Before attaching the case top, be sure to reconnect thegreen/yellow wire (attached to the case top), to the othergreen/yellow wire (attached to the case bottom), to ensureboth metal enclosures are properly grounded.5-5