Retriever IV Fraction Collector User Manual - Isco

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Retriever IV Fraction CollectorSection 4 Theory of OperationWhen the void start button (S102) is pushed, it will reset the voidvolume flip-flops (U119), and forces the Q output of U119A high.When Q goes high, U102G is turned on, DS401 lights up, andsimultaneously the security valve circuit (U108A, U104C,U102A, B) will turn off the valve. The Q output will remain highuntil the red rack has passed the sensor and the next tubeposition is reached.The security valve has another input which comes from themotor control flip-flop (U114B) Q. This Q output is appliedthrough U107C to the security valve drive (U108A, U104C,U102A, B) and to the pump stop signal drive (U102F). As thefraction collector advances, the security valve drive U102A andU102B will provide an open collector output on pin 12 of themonitor jack (J9) and to pin 1 of the valve jack (J10) to turn on/offa security valve. U102F provides an open collector output on pin5 of the count jack (J8) to stop a pump during tube changes.The monitor jack (J9) provides both a rack and a tube eventmark and an external advance signal. The tube event mark iscreated by U103 and U102D on pin 5. Each time the fraction collectortime/count reaches the preset number and sets the delaycontrol flip-flop U114A, an event mark occurs. The rack eventmark signal is created by U104A on pin 4. Each time the fractioncollector changes to the next consecutive rack, an event markoccurs. An external advance signal can be created on pin 2 with acontact closure or open collector. This signal is inverted byU131A and applied through U130A to U114A in the samemanner as a pulse from the time/count BCD switches (S104),resulting in starting the delay time and then moving to the nexttube position.Other signals on the monitor jack are motor stop, end of run, andpause count signal. An external motor stop signal can be createdon pin 11 with an open collector. This signal will keep U130Breset and trigger U129, resulting in keeping the motor fromturning on and advancing to the next tube. The end of run signalis created on pin 14. This end of run signal will turn on an opencollector output (U102C) when the red stop rack is reached. Theexternal pause count signal can be created on pin 13 with anopen collector. This signal will go to U106A and keep any countsfrom getting to the BCD counters.When power is initially applied to the circuitry, a capacitor(C130) is charged through U123H. During the brief chargingtime U123H’s output reaches high. This high signal will gothrough U101C, U113A and D setting U114B resulting in anadvance of the tube by one position. This occurs when the unit isfirst switched to operate and whenever power is restored after aninterruption assuring that collection starts or resumes in anempty tube.If the unit is powered up and the rack is in the stop position, anRC network on pin 5 U115B prevents the fraction collector fromadvancing past the stop position.4-4
Retriever IV Fraction CollectorSection 4 Theory of OperationWhen the last position on the red stop rack is reached, the outputof U127D will go low resetting the motor control flip-flop U114Bto stop the motor. In addition, the output of U115A will set theend of run flip-flop U128A. When U128A is set the Q output goeslow preventing any additional input counts on U105, also turningthe security valve output off, the pump stop output of the countconnector on, and operating the pump isolation relay K101. Thisresults in turning off any pumps connected to the fraction collectorand closing the security valve.4.1.1 Timing Diagram To illustrate the timing and relationship of signals during operation,selected waveforms are shown on the timing diagram,Figure 4-2. The timing diagram assumes the front panel switchesare set as shown below.TIME/COUNT - set at 001X1 /X10 - set XlTIME/VOL/DROP - set at TIMEDELAY/TIME - set at 004Figure 4-2 Timing Diagram4-5
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