Huygens Professional User Guide

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The easy way18 The Huygens Professional User Guide
CHAPTER 3Establishing imageparametersImage sizeComputing time. The amount of computing time involved in deconvolving images is morethan proportional to the image size. It is therefore sensible to limit the data size as much as possible.With WF images we recommend to not record planes below and above the object whichonly contain blur. Huygens Pro does not need these planes to restore your object. Since the blurin these planes might be affected by hard to correct bleaching they might even reduce the qualityof the deconvolution result.Brick wise processingComputer memory. Deconvolving images requires much computer memory because all computationsare done in 32-bit floating point format, and because several extra (hidden) imagesare needed to store intermediate results. To reduce the memory requirements Huygens Pro willsplit your images into bricks, deconvolve the bricks sequentially, and fit the bricks together in aseamless fashion. Brick wise processing is an automatic feature of Huygens Pro.The Huygens Professional User Guide 19
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Page 4 and 5: Copyright © 1997-2006 by Scientifi
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Page 8 and 9: Installation and system requirement
Page 10 and 11: Installation and system requirement
Page 12 and 13: Deconvolving my first imageFigure 2
Page 14 and 15: Deconvolving my first imageHistogra
Page 16 and 17: Deconvolving my first imageStep 4:
Page 18 and 19: The easy way• Press the Run butto
Page 20 and 21: The easy way• Destination of the
Page 24 and 25: original imageFigure 13. Example of
Page 26 and 27: ecome asymmetric at depths of alrea
Page 28 and 29: d2rd 2=πr 2 dand thus r = ------π
Page 30 and 31: Biorad confocal microscopesTABLE 6.
Page 32 and 33: sTCh_t01_z002_ch01.tifsTCh_t01_z003

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