Huygens Professional User Guide

eported by Huygens Pro is a measure relative to the first estimate and therefore a numbergreater or equal to 1. If the increase in quality drops below a threshold the iterations arestopped.Can I deconvolve aTiff series?Tiff file series namingconventionYes, if the series is a numbered series like: slice001.tif, slice002.tif.....slice0NN.tif, HuygensPro will read the series into a single 3D image. Because Tiffs usually carry no additionalmicroscopic information, check the parameters carefully.If you have Tiff images to be read into the Huygens Pro or the Huygens Essential you shouldknow about the naming convention used.If you select a file from a numbered series, the selected file and the following files will be interpretedas x-y planes of a 3D stack and read into a 3D image of suitable size and channel configuration.A one-channel 3D images only go with numbers:As an example a dataset called ‘c’ with 32 slices numbers as follows:c000.tifc001.tif..............c031.tifIf you wish to work on the complete c stack you only have to select ‘c000.tif’ while opening. Ifyou select file ‘c020-tif’ the first 20 slices will be skipped.Numbered series without the Tiff extension likec04c05...c18are not read in as a series.Huygens Essential and Professional read and write Tiff series with Leica style numbering ifthere are more channels (different wavelenghts), slices or frames(in time).An image of four slices and two frames is named with Leica style numbering as follows:c_t00_z000.tifc_t00_z001.tifc_t00_z002.tifc_t00_z003.tifc_t01_z000.tifc_t01_z001.tifc_t01_z002.tifc_t01_z003.tifAnd an image ‘sTCh’ of four slices, three frames and two channels:sTCh_t00_z000_ch00.tifsTCh_t00_z000_ch01.tifsTCh_t00_z001_ch00.tifsTCh_t00_z001_ch01.tifsTCh_t00_z002_ch00.tifsTCh_t00_z002_ch01.tifsTCh_t00_z003_ch00.tifsTCh_t00_z003_ch01.tifsTCh_t01_z000_ch00.tifsTCh_t01_z000_ch01.tifsTCh_t01_z001_ch00.tifsTCh_t01_z001_ch01.tifsTCh_t01_z002_ch00.tifThe Huygens Professional User Guide 27