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Herpetological Review Herpetological Review - Doczine

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FIG. 1. Mean (+ SE) number of turtles captured/pond during 13 days oftrapping (A = Trachemys scripta elegans; N = 7 ponds; B = Chrysemyspicta bellii; N = 8 ponds) with canned creamed corn, canned mackerel,and frozen fish. Means sharing the same lower case letter were not significantlydifferent (Fisher’s Protected LSD; α = 0.05).type to the three frame nets within a pond was random. Thereafter,frame nets were checked daily and the position of the PVCtubes containing the different baits systematically rotated dailyamong the three frame nets. The purpose of systematically rotatingthe baits among the three traps within each pond was to equallydistribute the possible influence of differences in capture ratesbetween specific trap locations within a given pond. Baits wereremoved from the perforated PVC tubes and replenished with freshbait every other day.Frame nets were set in 1–3 ponds/day over the 5 d period thatbegan 14 May 2007. We reversed this staggered schedule for removalof frame nets at the end of the 13 d study period to maintainan approximately equal number of trap hours in all ponds. Thus,all ponds were sampled continuously for a total of 13 d with substantialtemporal overlap of the trapping schedules (i.e., all trappingconducted from mid- to late May). There were 3 traps/pondfor 13 d in eight ponds (overall, 7488 trap hours summed acrossall three baits, 2496 trap hours/ bait, 936 trap hours/pond, and 312trap hours/pond/bait).All C. p. bellii and T. s. elegans captured were uniquely markedusing the system described by Cagle (1939). We considered allcaptures (original captures and recaptures) as independent in statisticalanalyses.Trap success may have been influenced by inherent site-specificvariation among the eight ponds and/or the temporal variationthat may have resulted from slight differences in the precisetrapping schedules among sites. Because of possible site-specificvariation, we used Analysis of Variance (ANOVA) within a randomizedblock design to compare the mean number of turtles (ofeach species) captured with each of the baits during the 13 d studyperiod. Blocking designs are desirable in such situations becausethey increase the precision of the model by removing one sourceof known (or suspected) variation (e.g., inherent site-specific variationin capture rates among ponds) from experimental error(Peterson 1985; Sokal and Rohlf 1995). The eight study pondswere considered as blocks, the different baits were the treatments,and mean number of turtles (i.e., either C. p. bellii or T. s. elegans)captured/pond with each of the three baits served as the responsevariable. Separation of means was accomplished using Fisher’sProtected Least Significant Difference (LSD; Peterson 1985) andalpha was set at 0.05 in all statistical tests.Results.—We captured 93 (69 original captures + 24 recaptures)C. p. bellii and 81 (50 original captures + 31 recaptures) T. s. elegansduring the 13 d study period. We captured C. p. bellii in all pondsand caught T. s. elegans with one or more of the baits in all but onepond. Therefore, we excluded this pond from the analyses for T. s.elegans. We observed a significant bait effect for both species (T.s. elegans: F = 5.25; d.f. = 2, 12; P = 0.023; R 2 = 0.69; C. p. bellii:F = 4.73; d.f. = 2, 14; P = 0.027; R 2 = 0.75). We captured significantlymore T. s. elegans in frame nets baited with frozen fish thanwith creamed corn, but observed no significant difference betweenfrozen fish and canned mackerel or between canned mackerel andcreamed corn (Fig. 1A). Mean separation procedures for C. p. belliirevealed that both canned mackerel and frozen fish were significantlymore effective than creamed corn but canned mackerel andfrozen fish were not significantly different from each other (Fig.1B).Discussion.—We concluded that both canned mackerel and frozenfish were significantly “better” than creamed corn for attractingthese two species to frame nets. First, we discuss several potentiallyconfounding factors that are known to influence capturerates. For example, captured females may serve as an additionalenticement for males to enter nets (Frazer et al. 1990; Jensen 1998;Thomas et al. 1999). However, we did not observe unusually largenumbers of males within traps containing females (see Jensen1998), and we see no reason to expect that the particular food baitwithin a trap would alter the attractiveness of a female within thattrap. Therefore, the influence of this factor should have been equalacross all three baits. Likewise, individual turtles sometimes exhibitso-called “trap-happy” or “trap-shy” behaviors (Deforce etal. 2004; Koper and Brooks 1998). But, we do not think that theobserved differences were an artifact of such behaviors. First, becauseof the short duration of our study period many individualswere never recaptured (i.e., captured only once) and most of thosethat were recaptured were recaptured only once. Second, turtleswere not rewarded for entering a trap (i.e., not allowed to consumethe baits) and the bait tubes were systematically rotated everyday among the three traps within each pond. Therefore, wehave no reason to expect the propensity for “trap-happiness” or“trap-shyness” to have differed among the three baits. Therefore,the influence of such behaviors (if any) should have been equalacross all three baits.Consistent with previous studies, we found that using different<strong>Herpetological</strong> <strong>Review</strong> 39(2), 2008 187

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