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ecover skin cells (Boyle et al. 2004;Retallick et al. 2006); each frog was stroked20 to 30 times on the abdomen, pelvic patch,inner thighs and in between toes or, in thecase of larvae, the oral disk was rubbed 5times in a circular motion with a sterile cottonswab. The swab was then placed individuallyin a 1.5 ml microfuge tube containinga DNA extraction buffer (Zolan andPukkila 1986) and stored at ambient temperature.All samples were processed twiceby JEJ using polymerase chain reaction(PCR) assay to detect Bd (Boyle et al. 2004;Annis et al. 2004). Individual animals werehandled with clean latex gloves or a plasticbag inverted over the observers hand. Equipmentwas sprayed with a concentrated sodiumhypochlorite solution between eachsite. Malformed metamorphs were collected,preserved in a 95% ethanol solutionat ambient temperature and x-rayed by a veterinarianto determine if malformationswere due to injury or amputation (SummitIndustries, Innovet Classic, ModelLX125V).Results.—We found Wood Frogs at 20 of26 known sites near Wonder Lake (Fig. 1).Frogs were not found at additional sites searched between the Parkentrance and the Savage River or in the Teklanika vicinity, althoughthey were not previously known to be present and may notoccur at these sites. Thirty frogs were swabbed, 12 at roadsideFIG. 1. Denali National Park (DNP), Alaska, USA, and Wood Frog (Rana sylvatica) Bd samplinglocations in the Wonder Lake vicinity.sites and 18 at backcountry sites up to 4.5 km from the road (Table1). No Bd was detected. Nearly 87% of the animals sampled werenewly metamorphosed. At one backcountry site, five (16%)metamorphs were malformed and had missing limbs, and threeTABLE 1. Bd was not detected in 30 wood frogs (Rana sylvatica) at Denali National Park, Alaska, USA, in 2006.Date Latitude Longitude Number Life Stage Condition Bd Detected?Observedof Animal(s)8 Aug 06 N63 26.960 W 150 52.169 1 Metamorph Normal No8 Aug 06 N63 26.248 W 150 53.979 7 Metamorph 4 Missing Limb, 3 Dead No9 Aug 06 N63 25.826 W 150 56.670 1 Metamorph Normal No10 Aug 06 N63 26.196 W 150 53.856 2 Adult Normal No10 Aug 06 N63 26.197 W 150 53.857 1 Metamorph Normal No11 Aug 06 N63 27.212 W 150 51.760 1 Metamorph Normal No12 Aug 06 N63 28.041 W 150 50.481 2 Metamorph Normal No14 Aug 06 N63 28.103 W 150 50.551 1 Metamorph Normal No14 Aug 06 N63 28.694 W 150 51.241 3 2 Metamorph, 1 Larvae Normal No14 Aug 06 N63 28.834 W 150 51.621 1 Metamorph Normal No14 Aug 06 N63 28.824 W 150 51.612 2 Metamorph Normal No14 Aug 06 N63 29.151 W 150 51.828 1 Metamorph Normal No14 Aug 06 N63 27.280 W 150 53.086 1 Adult Missing Limb No15 Aug 06 N63 27.279 W 150 51.806 1 Metamorph Normal No15 Aug 06 N63 27.371 W 150 53.327 1 Metamorph Normal No15 Aug 06 N63 27.485 W 150 53.706 1 Metamorph Normal No15 Aug 06 N63 27.652 W 150 53.528 1 Metamorph Normal No15 Aug 06 N63 27.646 W 150 53.402 2 Metamorph Normal NoHerpetological Review 39(2), 2008 203