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Comprehensive Report

GPO-DUELFERREPORT-3

GPO-DUELFERREPORT-3

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Figure 3 (b). Trailer 1 data plate on reactor vessel.<br />

Figure 3 (a). Trailer 1 reactor vessel.<br />

Figure 4. Trailer 1 box for holding product ‘K’ bottles.<br />

Comment and assessment<br />

• The simple filter F-2 is not of a design that will<br />

provide sterile compressed air to the process. A<br />

HEPA filter could be fitted to address this problem,<br />

although this would not be a minor modification.<br />

• Large amounts of compressed air are required for<br />

each production cycle (see 2.6) and the risk of contamination<br />

(failure) of any given batch is increased<br />

if process air is not sterilized.<br />

• The low pressure compressor set is of standard<br />

reciprocating design and will not provide “oil-free”<br />

air. Oil-free air compressors are often specified in<br />

fermentation installations though in this particular<br />

concept (i.e. the production of “fire-and-forget” BW<br />

agents), it is unlikely to be a critical factor.<br />

• These trailers were not designed with anaerobic fermentations<br />

in mind. Although it would be possible<br />

(but extremely inconvenient) to connect nitrogen<br />

cylinders to the sparge system, it is noteworthy that<br />

no provision has been made on the trailers for the<br />

84

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