conservation, characterisation and management of grapevine genetic

RESULTS
Molecular data on varieties
Until now, 2624 coded profiles were obtained. A preliminary population analysis was
performed based on the coded data produced during the project by all the partners at 9
microsatellite loci, including identical genotypes (Tab. 1) . The number of different alleles
(Na) found within the 47 reference cultivars (cv-refer in Table 1, similar to those analyzed by
This et al, 2004) was compared with the number of different alleles found in the population of
new and old genotyped accessions. Observed heterozygosity (Ho) was compared with
expected heterozygosity (He) at the same loci.
Table 1. Summary of SSR data available in the project
Locus
Ho (cv-
He (cv-
N (cv-refer) N (wp1) Na (cv-refer) Na (wp1) refer) Ho (wp1) refer) He (wp1)
VVMD5 47 2568 17,000 23,000 0,894 0,835 0,907 0,853
VVMD7 47 2595 17,000 24,000 0,809 0,818 0,861 0,816
VVMD25 47 2488 14,000 24,000 0,851 0,796 0,847 0,791
VVMD27 47 2624 23,000 28,000 0,979 0,841 0,898 0,840
VVMD28 47 2492 18,000 34,000 0,979 0,872 0,923 0,895
VVMD32 47 2441 14,000 40,000 0,511 0,803 0,883 0,832
VVS2 47 2586 19,000 27,000 0,894 0,792 0,889 0,818
VrZAG62 47 2580 19,000 25,000 0,872 0,833 0,885 0,827
VrZAG79 47 2545 13,000 19,000 0,787 0,814 0,882 0,861
Na = Number of alleles; Ho = observed heterozygosity, He = expected heterozygosity =
diversity measure
Morphological data on varieties
The growing database contains 1600 accessions characterized for 48 OIV descriptors (699
accessions described in GrapeGen06). Some key characters have been chosen to show the
variability among varieties and regions (Tab. 2).
Berry colour has a high incidence on wine organoleptic characteristics. The most frequent berry
colour is green-yellow (50 %) followed by black or violet colour (41 %) and other minor colours
like rose or green. But the situation is different among regions being dark colours predominant in
accessions studied by partners from Italy and Germany meanwhile green-yellow colours are
predominant in accessions from Austria, Portugal and all the Eastern Countries.
Bunch length is an indicator of bunch size and weight. The most common length is medium (55
%), but the low length is more frequent in accessions studied by France and Eastern Countries
(except Hungary) meanwhile bunches of higher length are clearly more frequent in accessions
from Germany.
The time of bud burst is an indicator of resistance to spring frost. Although many varieties have
the bud burst in a medium time, there are also many of them, which do it in early time (mainly in
France, Austria, most Eastern Countries, and some partners from Italy).
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