Abstracts 4. Gemeinsamer Jahreskongress der ... - SWISS KNIFE

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swissknife spezial 06 12.06.2006 13:39 Uhr Seite 36 red at the first dressing change, 2-3 days after trauma. VAC ® and Epigard ® -fluid was squeezed out of the foam and centrifuged at 2000g, 4°C for 20min, additionally patient’s serum was collected. Wound fluid, or patient’s serum was diluted with RPMI 1640 medium 1:10 (v/v) and incubated with freshly isolated PMN from healthy volunteers (106/ml) for 16 hours. Apoptosis of PMN was detected by flowcytometry after staining with Annexin-V-FITC and propidium iodine. Data are given as mean ± SEM; significance level was set at p < 0.05 using two-tailed Student’s-t-test. Results: Incubation of PMN from healthy volunteers (n=12) with VAC ® -fluid significantly inhibited spontaneous apoptosis compared to Epigard ® -fluid (10.8 ± 1.2% vs. 26.6 ± 1.8%, p < 0.05), where the apontaneous apoptosis was (47.3 ± 3.2%). Icubation of PMN with serum from VAC ® or Epigard ® treated patients led not to a significant change in PMN apoptosis rate. Conclusion: Increased formation of granulation in VAC ® -treated wounds contributes to better wound healing. Besides an induction of inflammation in the wound the continuous negative pressure generated by the VAC ® -system seems to induce a local inflammation with an accumulation of neutrophils improving the wound healing. 17.07 L. Sun1 , T. Reding1 , M. Bain1 , U. Ungethüm1 , F. Storni1 , P. Clavien2 , D. Bimmler3 , R. Graf1 1Dept. of Visceral and Transplant Surgery, University Hospital of Zurich, 8091 Zurich/CH, 2Swiss Hpb Center, Dept. Visceral and Transplant Surgery, University Hospital of Zurich, 8091 Zurich/CH, 3Chirugie, Praxis, 8001 Zürich/CH Prostaglandin E2 and TN synergistically promote the expression of monocyte chemotactic protein-1 in the pancreatic acinar cell AR42J: interaction between macrophages and pancreatic acinar cells in pancreatitis Objective: MCP-1 is a strong chemoattractant for monocytes, macrophages and lymphocytes, and plays an important role in the recruitment of monocytes/macrophages in inflammatory tissues. In a model of chronic pancreatitis (WBN/Kob rat) we recently demonstrated that the inhibition of COX-2 activity reduces and delays inflammation. The expression of MCP-1 was significantly diminished in COX-2 inhibitor treated rats as compared to untreated WBN/Kob rats. There was a markedly decreased level of PGE2 and decreased infiltration rate of macrophages in inhibitor treated rat pancreas. In this study, we investigated the regulation of pro-inflammatory molecules (PGE2, MCP-1and TNFï¡ © during interactions of pancreatic acinar cells and macrophages ® Methods: We first identified the cells which express MCP-1 in the rat model, and found it predominantly in acinar cells. To simulate interactions of macrophages and acinar cells, we used a mouse macrophage cell line (RAW 264.7) and a rat acinar cell line (AR42J). To test whether PGE2 might have any effects in acinar cells we first verified that PGE2 receptors EP1-4 are actually expressed in AR42J cells. The expression and regulation of MCP-1 in the pancreatic acinar cell line in response to PGE2 was then examined, and the migration of macrophages in response to pancreatic acinar secretory products was tested. Results: Pancreatic acinar cells produced MCP-1, and the expression of MCP-1 was regulated by TNFï¡ and PGE2. In the presence of PGE2, TNFï¡ dependent expression of MCP-1 was significantly higher than with TNFï¡ alone (p
swissknife spezial 06 12.06.2006 13:39 Uhr Seite 37 rative dietary supplementation with n-3 PUFAs protects macrosteatotic livers against ischemic injury via improving microcirculation. However, protective strategies applied during or after ischemia are unlikely to be successful. 17.12 M. Cikirikcioglu 1 , G. Bowlin 2 , Y.B. Cikirikcioglu 1 , J. Tille 3 , A. Kalangos 1 , B.H. Walpoth 1 1 Department of Cardiovascular Surgery, University Hospital of Geneva, 1211 Geneva/CH, 2 Biomedical Engineering, Virginia Commonwealth University, Virginia/US, 3 Department of Clinical Pathology, University Hospital of Geneva, 1211 Geneva/CH Degradable synthetic (PDS-based) electrospun scaffolds: assessment as an arterial graft in the rat model Objective: Vascular tissue engineering necessitates a degradable scaffold. The purpose of this study was to evaluate biocompatibility and thrombogenicity of electrospun polydioxanone(PDS) grafts. Methods: Electrospun PDS was selected because of biomechanical strength, surgeon’s handling, degradation, cellular ingrowth and tissue reaction in a subcutaneous implantation model. In 15 Sprague Dawley rats, 1-mm e-PTFE grafts (n=6) and 1-mm PDS grafts (n=9) [bare, external wrapped or mixed with poly(lactic acid) (PLA)] were interposed in the infrarenal abdominal aorta. Terminal digital substraction angiography was performed for patency assessment and grafts were harvested for morphologic as well as scanning electron microscopic examination. Results: At three weeks, PDS showed comparable patency rates (overall, 89%) to e-PTFE grafts (83%). However, saccular aneurysm formation was found in all unwrapped patent PDS grafts. This was prevented with external wrapping (3 grafts were wrapped with non-degradable woven matrix, e-PTFE or external electrospun nylon) or blend of PDS with PLA (n=3). At 6 and 12 weeks patency was 100% and aneurysmal dilation 30%. Conclusion: Patency of electrospun PDS 1-mm grafts is excellent. If the prosthesis was manufactured with PDS alone, aneurysm formation was seen in all cases. External wrapping or blending PDS with PLA seemed to prevent this complication at 3-weeks. However, at 6 and 12 weeks aneurysmal dilation was found in 30%. Therefore, combinations or wrapping of the grafts with stronger polymers may be required. In conclusion, electrospun PDS-based polymers may be promising materials for vascular tissue engineering. 17.13 P.C. Nett 1 , D. Stroka 1 , D. Candinas 2 1 Clinic of Visceral- and Transplant Surgery, University Hospital of Berne, 3010 Berne/CH, 2 Visceral and Transplantation Surgery, University Berne; Inselspital, 3010 Berne/CH Hypoxia driven endothelin receptor ETRA and ETRB expression is dependent on cellular differentation in pancreatic carcinoma Objective: Endothelin (ET), a potent endogenous vasoconstrictor and mitogen, is supposed to contribute to proliferation of tumour cells and to tumour angiogenesis. The pathway by which tumour cells produce increased levels of ETs has not been completely elucidated, but appears to involve transcriptional regulation of its two specific cell surface receptors (ETR), ETRA and ETRB, and hypoxia that induces angiogenesis. To determine whether gene expression of ET and its receptors is regulated by hypoxia in pancreatic adenocarcinoma, we studied their transcriptional activity in a model of human pancreatic adenocarcinoma under normoxic and hypoxic conditions. Methods: Two human pancreatic adenocarcinoma cell lines PANC-1 (poorly differentiated) and CAPAN-1 (moderately differentiated) were cultured under hypoxic (1.5% oxygen) or normoxic (21% oxygen) conditions for 48 hours. Total RNA was isolated and and cDNA was synthesized. mRNA expression of human ET-1, ET-2, ET-3, ETRA and ETRB as well as endothelin converting enzyme (ECE)-1, ECE-2, were analyzed by semi-quantitative PCR. Results: There was constitutive mRNA expression of all the genes tested in both PANC-1 and CA-PAN-1 cells. The transcriptional activity of ET-3 was greater under both normoxic and hypoxic conditions (p
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