Abstracts 4. Gemeinsamer Jahreskongress der ... - SWISS KNIFE

swissknife spezial 06 12.06.2006 13:40 Uhr Seite 59
Inhibiting CD26/dipeptidyl-peptidase IV intragraft activity attenuates posttransplant pulmonary
ischemia-reperfusion injury after extended ischemia
Objective: To investigate the effect of enzymatic DPP IV inhibition on ischemia-reperfusion
(I/R) injury after extended ischemia prior to transplantation.
Methods: A syngeneic rat orthotopic left lung transplantation model was used. In the control
group (group I), donor lungs were flushed and preserved in Perfadex ® for 18 hours at 4°C and
then transplanted and reperfused for 2 hours; Two treatment groups were tested: Group II
donor lungs were perfused with and stored in 25umol/L Pro-Pro-diphenyl phosphonate
(AB192), a small molecular weight DPP IV inhibitor; Group III donor lungs were only perfused
with AB192, and not stored in the presence of the inhibitor. We measured peak airway pressure
(PAwP) during the whole 2-hour reperfusion period, after 2-hour reperfusion, blood gas
analysis was measured, and graft wet/dry weight ratio, myeloperoxidase activity (MPO) and
thiobarbituric acid reactive substances (TBARS) were measured.
Results: Grafts from group II, group III vs. group I showed a significantly increased oxygenation
capacity (II: 330.4 ± 106.9 mmHg, III: 304.3 ± 113.3 vs. I: 138.5 ± 61.0, p