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pH-Assisted <strong>Thermal</strong> <strong>Processing</strong> 587<br />

At present it is well known that the majority of the microorganisms show<br />

their maximum heat resistance at neutral pHs, decreasing with an increase in<br />

acidification. The magnitude of the effect of pH will depend on the microbial<br />

species and other factors, such as food composition, type and concentration of<br />

acid, or even the sporulation temperature in the case of bacterial spores.<br />

pH influences not only heat resistance, but also bacterial growth, and most<br />

importantly, it influences very much the growth of the survivors to a heat treatment.<br />

Hence, the combined effect of the pHs of the heating medium and recovery<br />

medium may lead to synergistic reductions of the viability of microbes.<br />

The huge amount of data on the effect of pH on microbial growth and heat<br />

resistance has allowed for the development of large databases and mathematical<br />

models that enable prediction of the behavior of microorganisms under certain<br />

conditions. These data sets are available for food microbiologists, manufacturers,<br />

risk assessors, and legislative officers.<br />

REFERENCES<br />

1. WD Bigelow, JR Esty. The thermal death point in relation to time of typical<br />

thermophilic organisms. Journal of Infectious Diseases 27: 602–617, 1920.<br />

2. JR Esty, KF Meyer. The heat resistance of spores of B. botulinus and allied<br />

anaerobes. Journal of Infectious Diseases 31: 650–653, 1922.<br />

3. P Sognefest, GL Hays, E Wheaton, HA Benjamin. Effect of pH on thermal process<br />

requirements of canned foods. <strong>Food</strong> Research 13: 400–416, 1948.<br />

4. JJ Powers. Effect of acidification of canned tomatoes on quality and shelf life.<br />

Critical Reviews in <strong>Food</strong> Science and Nutrition 7: 371–396, 1976.<br />

5. DA Corlett Jr, MH Brown. pH and acidity. In: ICMSF, Microbial Ecology of<br />

<strong>Food</strong>s, Vol. I, Factors Affecting Life and Death of Microorganisms. New York:<br />

Academic Press, 1980, pp. 97–117.<br />

6. TC Odlaug, IJ Pflug. Clostridium botulinum in acid foods. Journal of <strong>Food</strong><br />

Protection 41: 566–573, 1978.<br />

7. AC Hersom, ED Hutland. Canned <strong>Food</strong>s. Edinburgh: Longman, 1980, pp. 80–115.<br />

8. G Cerny, W Hennlich, K Poralla. Spoilage of fruit juice by bacilli: isolation and<br />

characterization of the spoilage microorganisms. Zeitschrift fuer Lebensmittel<br />

Untersuchung und Forschung 179: 224–227, 1984.<br />

9. DF Splittstoesser, JJ Churey, CY Lee. Growth characteristics of aciduric sporeforming<br />

bacilli isolated from fruit juices. Journal of <strong>Food</strong> Protection 57:<br />

1080–1083, 1994.<br />

10. CA Wisse, ME Parish. Isolation and enumeration of sporeforming, thermoacidophilic,<br />

rod-shaped bacteria from citrus processing environments. Dairy, <strong>Food</strong> and<br />

Environmental Sanitation 18: 504–509, 1998.<br />

11. MNU Eiroa, VCA Junqueira, FL Schimdt. Alicyclobacillus in orange juice: occurrence<br />

and heat resistance of spores. Journal of <strong>Food</strong> Protection 62: 883–886, 1999.<br />

12. ML Fields, AF Zamora, M Bradsher. Microbiological analysis of home-canned<br />

tomatoes and green beans. Journal of <strong>Food</strong> Science 42: 931–934, 1977.<br />

13. TJ Montville. Metabiotic effect of Bacillus licheniformis on Clostridium botulinum:<br />

implications for home-canned tomatoes. Applied and Environmental Microbiology<br />

44: 334–338, 1982.

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