Biotechnology in Animal Husbandry - Institut za Stočarstvo

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Materials and Methods Study of biochemical polymorphism ... Electrophoresis. A polyacrylamide gel electrophoresis (PAGE) of transferrin protein fractions was carried out on 13-cm x 22-cm x 4-mm with 24 wells according to the method developed by Khaertdinov and Gataulin (2000). After applying an output voltage of 200 volts for 10 minutes, the inserts were removed and the same voltage continued for a further 15 minutes. The output voltage was then increased to 250 volts and continued until the brown line had migrated 9 cm beyond the insert line. The gel was then removed, sliced and stained for 10 min. with 0.1 % (w/v) Amido Black in methanol-acetic acid-water (50/7/43 by vol.). The gel was distained with a solution containing methanol-acetic acidwater (40/10/50 by vol.). Statistical analysis. The allele frequencies in the transferrin were estimated by direct counting of the phenotypes. To test differences between observed and expected genotypes frequencies, a chi-square (χ 2 ) analysis was performed on the basis of the Hardy-Weinberg law. Results and Discussion Figure 1 shows the electrophoretical patterns of some individual carp (Cyprinus carpio) protein samples. two bands were detected when transferrin was run and stained in Amido Black in methanol-acetic acid-water (50/7/43 by vol.). The Carp (Cyprinus carpio) transferrin types were named according to the nomenclature suggested by Irnazarow and Bialowas (1994) and Jurecka et al. (2009). The results obtained for the transferrin that show variation in the sample of Carp (Cyprinus carpio) are presented in Table 1. Gene frequencies were calculated by the method of gene counting as the mode of inheritance of each of the systems that do show variation is that of codominant alleles at an autosomal locus (Khaertdinov, 2000). Table 1. Distribution of transferrin Frequency and gene frequency transferrin locus in Carp (Cyprinus carpio) Transferrin genotypes, n= 116 χ 2 Gene frequency CC DD GG FF CD DG FD C D G F Number 8 45 23 24 8 8 15 21.54 0.12 0.50 0.12 0.26 % 6.72 37.82 6.72 20.71 6.72 6.72 12.61 The Carp (Cyprinus carpio) transferrin phenotypes are due to an autosomal locus with four co-dominant alleles, TfC , TfD, TfG and F. The D and F alleles were most frequent (0.5 and 0.26) respectively, while the C and G alleles were rare 267
Tf 268 T.A. Jaayid et al. alleles (0.12) (Figure 2). The gene frequency for D allele obtained in the sample is within the range of those observed in Jurecka et al. (2008), Csizmadia et al. (1995) and Wojtczak et al. (2007) while Valenta et al. (1976) have been found Seven transferrin variants (A,B,C,D,E,F, and G) in Carp. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Figure 1. Different transferrin genotypes as detected by polyacrylamide gel disc electrophoresis patterns at 8.6 in Iraqi Carp: 1-DG, 2-GG, 3-8-DD, 9-DG,10-DD, 11-FF, 12-13-DD, 14-GG, 15- FF, 16-CD, 17-FF, 18-CD.
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UDC 636 ISSN 1450-9156 VOL 27, 2 Fo
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Biotechnology in Animal Husbandry 2
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Table 1. Feeding regime durring the
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The effect of crossing of domestic
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152 Concentration of progesterone (
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154 J. Pleadin et al. Decision 2002
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158 J. Pleadin et al. CACCIATORE G.
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160 Efficiency of bovine IGF -I gen
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184 D. Vitorović et al. (Vićentij
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186 D. Vitorović et al. Broilers i
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188 D. Vitorović et al. deposition
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190 D. M. Ogah Blasco et al. (1984)
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192 D. M. Ogah Tabe 1. Descriptive
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194 D. M. Ogah chicken genotypes fo
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196 D. M. Ogah HU Y.H. (1999) .Vari
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198 Introduction V. Gerzilov et al.
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200 Results and Discussion V. Gerzi
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Results and Discussion Analysis of
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Analysis of applied biosecurity mea
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