Biotechnology in Animal Husbandry - Institut za Stočarstvo

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Efficiency of bovine IGF –I gene ... milk productivity animals using this experimental finding (marker-assisted selection). Table 1. The animal numbers and their accession numbers. Serial no. Animal no. Accession no. 1 2 gb|HQ183710| 2 1 gb|HQ183711| 3 4 gb|HQ183712| 4 9 gb|HQ183713| 5 8 gb|HQ183714| 6 6 gb|HQ183715| 7 16 gb|HQ183716| 8 3 gb|HQ183717| 9 43 gb|HQ183718| 10 30 gb|HQ183719| 11 36 gb|HQ183720| 12 46 gb|HQ183721| 13 38 gb|HQ183722| 14 45 gb|HQ183723| 15 48 gb|HQ183724| In 48 female Holstein cattle reared under Egyptian conditions, 249-bp of IGF-I gene was amplified and only in 15 animals (selected according to the highest and the lowest milk productivity) was sequenced. Table 2 shows the nucleotide sequence variation among these 15 animals in 249-bp of IGF-I gene, which was only in 12 nucleotides. As indicated, two single nucleotide polymorphisms (SNP) 163
164 Efficiency of bovine IGF –I gene ... at two different positions were found in one of the two highest milk productivity animals (animal number 1, BV = 11553). Where, all the 15 animals have adenine base (A) at the position 33 except animal number 1 has thymine base (T) at the same position (33). Also all the 15 animals have cytosine base (C) at the position 63 except animal number 1 has guanine base (G) at the same position (63), see Figure 2. In contrast, we could not identify any SNP in the other 14 highest and lowest milk productivity animals in these 12 nucleotide sequence variations. Thus, the present experiment showed that animals with T (SNP1) and G (SNP2) nucleotide sequence (33 and 63 positions) for the IGF-I gene can be used as marker-assisted selection (MAS) to select for high milk production trait. In previous related two studies to link between IGF-I gene polymorphisms and milk production trait, Siadkowsk et al. (2006) indicated effect of polymorphism in IGF1 gene on milk production trait in Polish Holstein-Friesian cattle. Where, restriction analysis of PCR-RFLP-SnaBI of the IGF1 gene (249-bp) showed three genotypes AA (223- and 26-bp), AB (249-, 26- and 223-bp) and BB (undigested band, 249-bp). Cows carrying AB genotype yielded more FCM, VCM milk, more milk fat and more milk protein than those AA and BB genotypes. Also, Mehmannavaz et al. (2010) studied IGF1 gene (249-bp) polymorphism and milk production trait in Iranian bulls. Digestion of the 249-bp PCR product with SnaB1 restriction enzyme (C-T substitution creates a SnaB1 restriction site) yielded three genotypes TT (223- and 26-bp), TC (223-, 26-, 249-bp) and CC (249-bp). Results revealed that bulls with genotype TC had higher estimated breeding values of milk and fat yield compared to CC and TT genotypes.
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Study of biochemical polymorphism .
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Physiological aspects of behaviour
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Instruction for authors Papers for
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PETROVIĆ D.M., GUTIĆ M., BOGOSAVL
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Registration fee Registration fee i
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