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Caracterización <strong>de</strong>l gen pnl 2 que codifica <strong>la</strong> pectín liasa 2 <strong>de</strong> Colletotrichum lin<strong>de</strong>muthianumniger are enco<strong>de</strong>d by a family of divergedgenes. Eur J Biochem 208:83–90.Cal<strong>de</strong>rón-Cortes, N. 2004. Detección <strong>de</strong>dos genes <strong>de</strong> patogenicidad a partird e u n a g e n o t e c a d e l h o n g oColletotrichum lin<strong>de</strong>muthianum raza1472. Tesis <strong>de</strong> licenciatura. Facultad<strong>de</strong> Biología. U.M.S.N.H.Dyrlov B. J., Nielsen, H., von Heijne, G.and Brunak, S. 2004. Improved predictionof signal pepti<strong>de</strong>s SignalP3.0. J Mol Biol 340:783-795Gysler, C., Harmsen, J.A.M., Kester,H.C.M., Visser, J. and Heim, J. 1990.Iso<strong>la</strong>tion and structure of the pectinlyase D-encoding gene from Aspergillusniger. Gene 89:101–108.Kozak, M. 1986. Point mutations <strong>de</strong>finea sequence f<strong>la</strong>nking the AUG initiatorcodon that modu<strong>la</strong>tes trans<strong>la</strong>tionby eukaryotic ribosomes. Cell44:283–292.Kupfer, M. D., Drabenstot, D. S., Buchanan,L. K ., Hua Zhu, L. H., Dyer, W.D., Roe, A. B. and Murphy, W. J.2004. Introns and splicing elementsof five diverse fungi. Euk Cell3:1088–1100.Kusters-van, Someten, M.A., Flipphi, M.,<strong>de</strong> Graaff, L., van <strong>de</strong>n Broeck, H.,Kester H, Hinnen, A. and Visser, J.1992. Characterization of the Aspergillusniger pelB gene: structure andregu<strong>la</strong>tion of expression. Mol GenGenet 234:113–120.Lara-Márquez, A. 2004. Construcción <strong>de</strong>una genoteca <strong>de</strong> Colletotrichumlin<strong>de</strong>muthianum para ais<strong>la</strong>r el genpectín liasa (pnl2). Tesis <strong>de</strong> licenciatu r a . Fa c u l t a d d e B i o l o g í a .U.M.S.N.H.Lara-Márquez, A. 2007. Caracterizaciónbioquímico-molecu<strong>la</strong>r <strong>de</strong> <strong>la</strong> enzimap e c t í n l i a s a 2 ( pnl2) d eColletotrichum lin<strong>de</strong>muthianum. Tesis<strong>de</strong> maestría. Instituto <strong>de</strong> Investigaciónen Biología Experimental.<strong>Universidad</strong> <strong>de</strong> Guanajuato.Mayans, O., Scott, M., Connerton, I.,Gravesen, T., Benen, T., Visser, J.,Pickersgill, R. and Jenkins, J. 1997.Two crystal structures of pectin lyaseA from Aspergillus reveal a pH drivenconformational change and strikingdivergence in the substrate-bindingclefts of pectin and pectate lyases.Structure 5:677-689.Pickersgill, R. W. and Jenkins, J. A. 2003.The structures and active sites of pectinases:Advances in pectin and pectinaseresearch. Kluwer Acad. Pub.The Nether<strong>la</strong>nds. 267-275.Ranveer, S. J., Shivalika, S. and Reena, G.2005. Microbial pectinolytic enzymes:a review. Proc Biochem40:2931-2944.Vitali, J., Schick, B., Kester, H.C.M., Visser,J. and Jurnak, F. 1996. The threedimensionalstructure of Aspergillusniger pectin lyase B at 1.7-A resolution.P<strong>la</strong>nt Physiol 116:69-80.Von Heijne. 1983. Patterns of amino acidsnear signal-sequence cleavagesites. Eur J Blochem 133:17-21.No. Especial 2008 Ciencia Nico<strong>la</strong>ita [117]

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