Susanna Tamman

SisukordLühendid ja mõisted ...................................................................................................................3Sissejuhatus ................................................................................................................................41 Kirjanduse ülevaade ................................................................................................................51.1 Klebsiella spp. üldiseloomustus .......................................................................................51.1.1 Morfoloogia ja füsioloogia ........................................................................................51.1.2 Perekond Klebsiella liigid .........................................................................................51.1.3 Perekond Klebsiella levik..........................................................................................61.2 Klebsiella pneumoniae .....................................................................................................61.2.1 Klebsiella pneumoniae ja haiglainfektsioonid...........................................................61.2.2 Patogeensusfaktorid...................................................................................................7Kapsel .............................................................................................................................7Fimbriad .........................................................................................................................81.3 Klebsiella pneumoniae antibiootikumiresistentsus ..........................................................91.3.1 β-laktaam-antibiootikumid ........................................................................................91.4 Klebsiella pneumoniae tüpiseerimismeetodid................................................................101.4.1 Seroloogiline tüpiseerimine.....................................................................................111.4.2 Pulssvälja geelelektroforees (PFGE).......................................................................121.4.3 PCR (ing. k. Polymerase Chain Reaction) põhised tüpiseermismeetodid ..............131.5 Klebsiella pneumoniae Eestis.........................................................................................172 Eksperimentaalosa.................................................................................................................182.1 Eesmärgid .......................................................................................................................182.2 Materjal ja metoodika.....................................................................................................192.2.1 Tüvede isoleerimine ja säilitamine..........................................................................192.2.2 Bakteritüvede samastamine .....................................................................................192.2.3 Bakteriaalse DNA molekulaarne tüpiseerimine pulssvälja geelelektroforeesimeetodil ............................................................................................................................202.2.4 Bakteriaalse DNA molekulaarne tüpiseerimine multiensüüm-multipleks AFLP-PCR meetodil....................................................................................................................202.3 Tulemused ja arutelu ......................................................................................................232.3.1 Isoleeritud tüved ......................................................................................................232.3.2 PFGE .......................................................................................................................242.3.3 Multiensüüm-multipleks AFLP-PCR......................................................................242.3.4 Patsientide koloniastsioon .......................................................................................272.4 Järeldused .......................................................................................................................29Kokkuvõte ................................................................................................................................30Summary...................................................................................................................................31Kasutatud kirjandus ..................................................................................................................322