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Proceedings of the Seventh Mountain Lion Workshop

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ecommendations for future use. We<br />

placed 4 GPS collars from Telemetry<br />

Solutions (1130 Burnett Avenue, Suite J,<br />

Concord, CA 94520) on pan<strong>the</strong>rs during<br />

our 2001-2002 capture season. Two<br />

were Posrec collars that stored data on<br />

board until <strong>the</strong> collar was retrieved and<br />

<strong>the</strong> o<strong>the</strong>r two were Simplex units that<br />

had <strong>the</strong> ability to transmit data for<br />

remote downloads as well as store-onboard<br />

capabilities. Additionally, each<br />

collar was equipped with a VHF beacon<br />

in order to detect and recover carcasses,<br />

pinpoint and visit dens, and enable<br />

comparisons between GPS locations and<br />

aerial VHF relocations. Each pan<strong>the</strong>r<br />

equipped with a GPS collar was located<br />

thrice weekly during our regularly<br />

scheduled telemetry flights. All GPS<br />

collars have been recovered and we are<br />

currently evaluating data and<br />

performance. Two pan<strong>the</strong>rs wearing<br />

Posrec collars died 7 months after<br />

deployment, one Simplex model<br />

failed completely after only 4 months,<br />

and <strong>the</strong> remaining Simplex’s main<br />

battery failed after 6 months, disrupting<br />

GPS capabilities, but VHF function was<br />

maintained through <strong>the</strong> back-up battery.<br />

A final report is scheduled to be<br />

completed by <strong>the</strong> end <strong>of</strong> 2003.<br />

Feasibility <strong>of</strong> Using Remote Cameras to<br />

Survey Florida Pan<strong>the</strong>rs<br />

Most <strong>of</strong> what is known about Florida<br />

pan<strong>the</strong>rs, including population<br />

demographics, has come from radiotelemetry<br />

studies over <strong>the</strong> past 20 years.<br />

However, standardized survey<br />

techniques that estimate pan<strong>the</strong>r<br />

population parameters with associated<br />

measures <strong>of</strong> statistical confidence and<br />

that document significant changes in<br />

<strong>the</strong>se parameters over time have not<br />

been applied. The objective <strong>of</strong> this study<br />

is to assess whe<strong>the</strong>r infrared-triggered<br />

camera surveys for pan<strong>the</strong>rs provide<br />

PROCEEDINGS OF THE SEVENTH MOUNTAIN LION WORKSHOP<br />

FLORIDA STATUS REPORT · Lotz and Land 21<br />

adequate data for inclusion into capturerecapture<br />

models based on <strong>the</strong> Lincoln-<br />

Peterson estimator. Remote camera<br />

surveys could complement existing labor<br />

and cost-intensive survey methodology<br />

to provide a more accurate estimate <strong>of</strong><br />

pan<strong>the</strong>r population parameters and<br />

document significant changes in <strong>the</strong>se<br />

parameters over time. Passive infrared<br />

cameras (Cam Trakker, CamTrak<br />

South Inc., Watkinsville, GA) were<br />

deployed systematically on two areas<br />

within <strong>the</strong> current occupied range <strong>of</strong><br />

Florida pan<strong>the</strong>rs. The Florida Pan<strong>the</strong>r<br />

National Wildlife Refuge provided an<br />

opportunity to assess camera survey<br />

methodology in a core area with a<br />

sample population <strong>of</strong> radio-collared and<br />

uncollared pan<strong>the</strong>rs. Long Pine Key<br />

within Everglades National Park<br />

provided an opportunity to assess<br />

camera survey methodology in a quasigeographically<br />

closed population <strong>of</strong><br />

radio-collared and uncollared pan<strong>the</strong>rs.<br />

Cameras were systematically placed in<br />

each study area and trials <strong>of</strong> 15 and 30<br />

days were run with 15 and 30 cameras<br />

per session. Field work was completed<br />

in 2002 and a final report is expected<br />

later this year.<br />

Feasibility <strong>of</strong> Extracting Florida Pan<strong>the</strong>r<br />

DNA from Scats<br />

Pan<strong>the</strong>r scats could potentially <strong>of</strong>fer <strong>the</strong><br />

safest and most cost effective tool for<br />

censussing numbers <strong>of</strong> pan<strong>the</strong>rs,<br />

measuring population genetic health, and<br />

identifying origins <strong>of</strong> Puma sign found<br />

outside <strong>of</strong> core pan<strong>the</strong>r areas. The<br />

purpose <strong>of</strong> this study is to evaluate <strong>the</strong><br />

use <strong>of</strong> pan<strong>the</strong>r scats as a source <strong>of</strong> DNA<br />

samples for on-going genetic<br />

monitoring. Existing tissue samples<br />

were used to calibrate and verify <strong>the</strong><br />

utility <strong>of</strong> extracting and analyzing DNA<br />

from scats. Scat collection routes were<br />

established along existing trails on four

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