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Proceedings of the Seventh Mountain Lion Workshop

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22 FLORIDA STATUS REPORT · Lotz and Land<br />

areas and regularly surveyed by ATV.<br />

Over 400 km <strong>of</strong> trail were surveyed with<br />

scats encountered every 45 km on<br />

average. Additionally, scats were<br />

collected opportunistically during o<strong>the</strong>r<br />

field activities. Results are currently still<br />

being analyzed but microsatellite<br />

amplification <strong>of</strong> Florida pan<strong>the</strong>r DNA<br />

was successfully extracted in 60% <strong>of</strong> <strong>the</strong><br />

samples. Although collecting pan<strong>the</strong>r<br />

scat is labor intensive, utilizing DNA<br />

extracted from Florida pan<strong>the</strong>r scat holds<br />

promise as an unobtrusive technique to<br />

monitor <strong>the</strong> genetic health and individual<br />

makeup <strong>of</strong> <strong>the</strong> population. A final report<br />

will be completed later this year.<br />

Pan<strong>the</strong>r Peripheral Area Survey<br />

The only verified breeding population <strong>of</strong><br />

Florida pan<strong>the</strong>rs is in <strong>the</strong> sou<strong>the</strong>rn<br />

portion <strong>of</strong> <strong>the</strong> state, south <strong>of</strong> <strong>the</strong><br />

Caloosahatchee River and Lake<br />

Okeechobee, in <strong>the</strong> Big Cypress and<br />

Everglades physiographic regions. This<br />

population has been growing since <strong>the</strong><br />

mid 1990’s and so far 3 radio-collared<br />

pan<strong>the</strong>rs have crossed <strong>the</strong> river.<br />

Additionally, three uncollared pan<strong>the</strong>rs<br />

have been verified north <strong>of</strong> <strong>the</strong> river in<br />

recent years: two by tracks and/or<br />

photos, <strong>the</strong> o<strong>the</strong>r was road-killed. All <strong>of</strong><br />

<strong>the</strong>se have been males that dispersed<br />

from <strong>the</strong> core population. Only three are<br />

presumed to still be alive. Since resident<br />

male pan<strong>the</strong>rs typically encompass<br />

several females within <strong>the</strong>ir territory it is<br />

hypo<strong>the</strong>sized that searching <strong>the</strong>se ranges<br />

will afford <strong>the</strong> best opportunity <strong>of</strong><br />

finding o<strong>the</strong>r pan<strong>the</strong>rs if <strong>the</strong>y exist. This<br />

5-year study to determine <strong>the</strong> occurrence<br />

and status <strong>of</strong> pan<strong>the</strong>rs on peripheral<br />

areas <strong>of</strong> <strong>the</strong>ir presently known range has<br />

entered its final year. Systematic sign<br />

surveys have been conducted in areas<br />

where two male pan<strong>the</strong>rs had established<br />

territories. No sign <strong>of</strong> o<strong>the</strong>r pan<strong>the</strong>rs<br />

PROCEEDINGS OF THE SEVENTH MOUNTAIN LION WORKSHOP<br />

was found. A final report is scheduled<br />

for 2003.<br />

Effect <strong>of</strong> Genetic Introgression on<br />

Prevalence and Intensity <strong>of</strong><br />

Gastrointestinal Helminth Infections in<br />

Florida Pan<strong>the</strong>rs<br />

The effects <strong>of</strong> genetic restoration <strong>of</strong> <strong>the</strong><br />

Florida pan<strong>the</strong>r are being examined on<br />

many fronts. Complementing o<strong>the</strong>r<br />

projects, this study will indirectly assess<br />

<strong>the</strong> suspected improvement in immune<br />

function in intergrades by comparing<br />

gastrointestinal tract parasite burdens to<br />

that seen in original Florida pan<strong>the</strong>rs.<br />

Concurrently we will assess <strong>the</strong> efficacy<br />

<strong>of</strong> field an<strong>the</strong>lmintic treatment <strong>of</strong><br />

pan<strong>the</strong>rs. Before introgression,<br />

gastrointestinal parasite burdens were<br />

assessed in 11original Florida pan<strong>the</strong>rs.<br />

Gastrointestinal tracts from a minimum<br />

<strong>of</strong> 7 pan<strong>the</strong>rs descended from Texas<br />

puma will be assessed by 2005 at which<br />

time a final report will be prepared.<br />

RECENT PUBLICATIONS<br />

CUNNINGHAM, M.W., M.R. DUNBAR, C.D.<br />

BUERGELT, B. HOMER, M. ROELKE-<br />

PARKER, S.K. TAYLOR, R. KING, S.B.<br />

CITINO, AND C. GLASS. 1999. Atrial<br />

septal defects in <strong>the</strong> Florida pan<strong>the</strong>r.<br />

Journal <strong>of</strong> Wildlife Diseases 35(3): 519-<br />

530.<br />

DEES, C.S., J.D. CLARK, AND F.T. VAN<br />

MANEN. 2001. Florida pan<strong>the</strong>r habitat<br />

use in response to prescribed fire.<br />

Journal <strong>of</strong> Wildlife Management 65:141-<br />

147.<br />

DUNBAR, M.R., M.W. CUNNINGHAM, AND<br />

S.T. LINDA. 1999. Vitamin A<br />

Concentrations in Serum and Liver from<br />

Florida Pan<strong>the</strong>rs. Journal <strong>of</strong> Wildlife<br />

Diseases 35(2): 171-177.<br />

JANIS, M.W. AND J.D. CLARK. 2002.<br />

Response <strong>of</strong> Florida pan<strong>the</strong>rs to<br />

recreational deer and hog hunting.

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