Årsrapport 2011 - Region Sjælland

F A C U L T Y O F H E A L T H S C I E N C E S
U N I V E R S I T Y O F C O P E N H A G E N
P 67
Background
Materials and Methods
Toft Kristensen T, Feldthusen A-D, Anthonsen S, Wilms L, Larsen J, Lyngsie Pedersen P, Kvetny J
Mitochondrial Research Unit, Naestved Hospital, Region Zealand, Denmark. Corresponding author: Tina Toft Kristensen, tikr@regionsjaelland.dk
Mitochondria produce energy for the cell by converting
oxygen and nutrients into ATP. Thyroid hormones have a
major influence on mitochondrial activity. The basal
oxygen consumption reflects the intracellular stimulation
of mitochondria by thyroid hormone. Expression rates of
PGC-1α, PGC-1β, NRF-2 and TFAM are believed to reflect
the genomic stimulation of mitochondrial activity and can
be measured in MNBCs (1). The MMP reflects the
functional status of the mitochondria and is believed to be
a measure of direct stimulation by thyroid hormone. The
MMP can be determined by flow cytometry analysis of
fluorescence of MTG-stained MNBCs (2;3). Euthyroid
values of serum TSH and fT3 are inversely correlated to
age in children and adolescents (4).
Aim of study
To examine whether thyroid hormone effect on mitochondrial function is related to age.
Lean female children (age group A: 9-13 years of age,
n=11, Body Mass Index < 80, age matched percentile),
lean female adolescents (age group B: 15-20 years of age,
n=9, Body Mass Index = 20-22 kg/m2) and lean female
adults (age group C: 32-52 years of age, n=19, Body Mass
Index= 21-24 kg/m2) from the pediatric outpatient clinic,
Naestved Hospital, Region Zealand, Denmark
participated in the study. Height and weight were
measured and analysis of TSH and free T3 was performed
by electrochemical luminescent immunoassay.
Results
Table 1
Genomic
Age group A Age group B Age group C Kruska-Wallis ANOVA
fT3 pmol/l 6.50 (5.80-7.10) 6.40 (6.10-6.65) 4.90 (4.50-5.60)
VO2 ml O2/min*m2 137 (134-157) 120 (113-121) 118 (112-128)
Direct effect
A vs C p