from indigenous fermented foods and human gut ... - Thapar University

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Chapter IV: Results
stemmed from Hukutti mass and children meconium exhibited characteristics typical of
Lactobacilli of the L. helveticus and L. fermentum-group respectively. These strains produced
gases from glucose and also fermented the pentose sugars tested (Table 4.3), indicating
obligately homofermentative Lactobacilli. Furthermore, they lacked mDAP in the cell wall
and produced DL- lactate (Table 4.3).
4.5 Enzyme assay
None of the six isolates produced enzymes such as alkaline phosphatase, esterase
(C4), esterase lipase (C8)-lipase (C14), trypsin, cystine acrylamidase, �-mannosidase, and �-
fucosidase (Table 4.4). However, leucin acrylamidase, valine acrylamidase, acid phosphatase,
naphthol-AS-BI-phosphohydrolase,�,�-galactosidase, �-glucosidase, and N-acetyl-�-
glucosaminidase were detected. The carcinogen enzyme, �-glucuronidase was not produced
by any of the isolated Lactobacilli.
Table 4.3 Enzyme profile of Lactobacillus spp.
Enzyme LAM-1 LAM- 2 LKH-2 LKH-3 LKH-5 Lamec-29
Control 0 0 0 0 0 0
Alkaline phosphatase 0 0 0 0 0 0
Esterase (C4) 0 0 0 0 0 0
Esterase lipase (C8) 0 0 0 0 0 0
Lipase (C14) 0 0 0 0 0 0
Leucine acrylamidase 5 5 5 5 5 2
Valine arylamidase 5 5 5 5 5 4
Cystine acrylamidase 0 0 0 0 0 0
Trypsin 0 0 0 0 0 0
�-Chymotrypsin 0 0 0 0 0 4
Acid phosphotase 2 2 2 2 2 5
Napthol-AS-
BI-phosphohydrolase
3 3 3 3 3 3
�-Galactosidase 4 4 4 4 4 4
�- Galactosidase 4 4 4 4 4 4
�-Glucuronidase 0 0 0 0 0 0
�-Glucosidase 4 4 4 4 4 2
N-acetyl-B- 2 2 2 2 2 2
�-Mannosidase 0 0 0 0 0 0
�-Fucosidase 0 0 0 0 0 0