from indigenous fermented foods and human gut ... - Thapar University

104
Chapter IV: Results
Fig. 4.7 Chloestrol removal by Lactobacillus strain. Each point represents the mean ±
standard error (SE) mean of three independent experiments.
4.10 Detection of antimicrobial peptide (bacteriocin)
A total of six Lactobacillus isolated from traditionally food and meconium was
evaluated for the production of bacteriocins or antimicrobial peptides. In the agar spot test,
the Enterobacter (Chronobacter) sakazakii ATCC 51329, L. monocytogenes ATCC 19111, S.
flexneri 2a, A. hydrophila ATCC 35654, Y. enterolitica ATCC 9610 and S. typhimurium
ATCC 19585 indicator strains showed weak to strong inhibition (zone of inhibition of more
than 1 mm from edge of producer colony up to 23 mm).The results are shown in Table 4.21,
which depecit strains with antagonistic activity, as demonastrable by the broad range of
pathogen inactivation profile. The bacteriocin elaborated by L. casei LAM-1 was judged to
be most potent, amongst the six isolates. The bacteriocin was thus further purified and
characterized.