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from indigenous fermented foods and human gut ... - Thapar University

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Glycerol in water 30.0%<br />

Staining solutions<br />

Alcian Blue 0.1%<br />

3% glacial acetic acid 100.0 mL<br />

DMEM used for culturing of Caco2 colon carcinoma cells. Dulbecco’s modified<br />

Eagle’s medium (Invitrogen Technologies, USA).<br />

DEPC-H 2 O. DEPC (diethylpyrocarbonate) 0.1 %<br />

The solution was agitated overnight <strong>and</strong> then autoclaved to eliminate DEPC. DEPC-H2O<br />

is free of RNases.<br />

Primers<br />

16S rDNA forward primer 5’-AGAGTTTGATCCTGGCTCAG-3’<br />

16S rDNA reverse primer 5’-ACGGGCGGTGTGTTC-3’<br />

e

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