from indigenous fermented foods and human gut ... - Thapar University

4.7.2 Survival of Lactobacillus strains in the presence of bile
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Chapter IV: Results
Gastrointestinal systems have varying concentrations of bile. The rate of secretion of
bile and the concentration of bile in different regions of the intestine vary, depending mainly
on the type of food consumed and it may not be possible to predict the bile concentration in
the intestine at any given moment. The viable count of six different Lactobacillus strains in
the bile concentrations of 0.3% was presented in Tables 4.14. All six Lactobacillus strains
showed different degrees of sensitivity towards this compound up to 3 h of incubation. LAM-
1 showed highest growth followed by Lamec-29 and LKH-2 at 0.3% bile concentration.
Remaining Lactobacillus strains were observed to have fair growth up to 3 h of incubation.
Results of 0.3% bile concentration were presented in (Table 4.14). The isolate LKH-5, LAM-
2 and LKH-3 showed reasonable growth during incubation.
Table 4.14 Ability of Lactobacillus strains to grow in MRS broth in the presence of bile
0.3% at 37°C
Viable count a (log10 CFU/ml)
Strains 0 h 3 h % inhibition b
LAM-1 9.83 ± 0.07 8.20 ± 0.39 16.58
LAM-2 9.06 ± 0.03 6.23 ± 1.12 31.29
LKH-2 9.21 ± 0.12 6.50 ± 0.47 15.39
LKH-3 9.81 ± 0.08 5.96 ± 0.36 39.2
LKH-5 9.96 ± 0.23 5.79 ± 0.81 41.9
Lamec-29 9.05 ± 0.08 7.96 ± 0.04 12.0
a log mean counts of three trials (average ± s.d.); -cno inhibition; n = 3; observations comes from three replicate assays; data are represented as mean ±
sd; b % inhibition = [(cfu/ml initial – cfu/ml final)/ cfu/ml initial] x 100