from indigenous fermented foods and human gut ... - Thapar University
from indigenous fermented foods and human gut ... - Thapar University
from indigenous fermented foods and human gut ... - Thapar University
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4.6 Molecular characterization of the bacterial isolates<br />
81<br />
Chapter IV: Results<br />
The 16S rDNA sequence of all the isolates was obtained by sequencing the 16S rDNA<br />
amplicon with the primers 16Sseqfw (5’-AGA GTT TGA TCM TGG CTC AG-3’) <strong>and</strong><br />
16Sseqrev (5’-GGN TAC CTT GTT ACG ACT TC-3’). Sequences of all the respective<br />
cultures were deposited in the GenBank database with accession number (Table 4.5). To<br />
identify these six bacterial isolates, all were subjected to 16S rRNA amplification using<br />
primers, <strong>and</strong> about 1.5 Kb amplicon was observed in all isolates (Fig. 4.2). 16S rRNA<br />
PCR products were sequenced using Applied Biosystems automatic sequencer.<br />
Sequencing reactions were performed with the primers.<br />
Fig. 4.2 16S rDNA amplification of bacterial isolates. Lane 1-7: LAM-1, LAM-2, LKH-<br />
2, LKH-3, LKH-5, HKT-9 <strong>and</strong> Lamec-29; Lane 8: Control <strong>and</strong> Lane M: 1 Kb marker.
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