from indigenous fermented foods and human gut ... - Thapar University
from indigenous fermented foods and human gut ... - Thapar University
from indigenous fermented foods and human gut ... - Thapar University
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4.13.3 Membrane permeability<br />
117<br />
Chapter IV: Results<br />
The fluorescence ratios of the nisin-treated samples were taken to represent 100%<br />
permeability in comparison with the untreated cells. Pathogen cell suspensions treated with<br />
bacteriocin showed an increase in membrane permeability after 5 min of exposure (35%<br />
increase), which remained unchanged after 60 min (Fig. 4.17 A & B).These results indicate<br />
that the bacteriocins may not be a pore-former per se like nisin, but it does appear to have a<br />
destabilizing effect on the integrity of the cell membrane.<br />
A B<br />
Fig 4.17(A & B) Live <strong>and</strong> dead cells of Salmonella typhimurium. A represents the cells<br />
treated with bacteriocin <strong>and</strong> B represents the untreated cells<br />
4.14 Inhibition of pathogen by bacteriocin in a simulated /laboratory prepared food<br />
matrix <strong>and</strong> following processing<br />
Storage of the active substance at 4°C for 15 days did not influence the activity<br />
bacteriocin were subjected to frozen at -20°C during 1 day <strong>and</strong> thawed for 20 min at 25°C.<br />
After three freezing <strong>and</strong> thawing cycles, in general, culture supernatants maintained similar<br />
antagonistic properties. The fact that bacteriocin demonstrated freezing <strong>and</strong> thawing stability<br />
has technological importance <strong>and</strong> may broaden the potential application of such compounds
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