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Bonetti et. al., PLoS Genet. 2010 May; 6(5): e1000966.
Figure 6 Nuclease requirements for ssDNA generation at native telomeres.
Figure 6: (A) G1-arrested cells were incubated at 37°C for 4 hours in the presence of αfactor.
Genomic DNA was analyzed as in Figure 5A. (B) Exponentially growing cells
were incubated at 37°C for 4 hours. Genomic DNA was analyzed as in Figure 5A.
(C,D) Genomic DNA prepared from exponentially growing cells at 25°C was analyzed
as in Figure 5A.
Similar to what we observed at native telomeres, 5′ C-strand degradation in
G1-arrested yku70Δ cells was abolished in the absence of Exo1, whereas it
occurred in yku70Δ mre11Δ cells (Figure 7A–7C). Conversely, the lack of Exo1
did not affect 5′ C-strand degradation in G1-arrested rif2Δ cells, where
degradation of the same strand was instead abolished in the absence of
Mre11 (Figure 7D–7F). Unfortunately, we were unable to synchronize rap1ΔC
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