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Anbalagan et. al., PLoS Genet. 2011 March; 7(3): e1002024 Acknowledgments We thank J. Diffley, J. Haber, D. Lydall, and V. Lundblad for providing yeast strains and antibodies. We thank Valeria Viscardi for some preliminary data, Marina Martina for technical support in performing the in-gel hybridization assay, Michela Clerici for critical reading of the manuscript, and all the members of the laboratory for helpful discussions. This work was supported by grants from Associazione Italiana per la Ricerca sul Cancro (grant IG5636 to MPL), Cofinanziamento 2008 MIUR/Università di Milano-Bicocca (to MPL), and Cofinanziamento 2007 MIUR/Università di Milano-Bicocca (to GL). DB and SA were supported by a fellowship from Fondazione Italiana per la Ricerca sul Cancro and by EU contract PITN-GA- 2008-215148 Image DDR, respectively. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. 86
References Anbalagan et. al., PLoS Genet. 2011 March; 7(3): e1002024 1. Longhese MP (2008) DNA damage response at functional and dysfunctional telomeres. Genes Dev 22: 125–140. 2. Nugent CI, Hughes TR, Lue NF, Lundblad V (1996) Cdc13p: a single-strand telomeric DNA-binding protein with a dual role in yeast telomere maintenance. Science 274: 249–252. 3. Evans SK, Lundblad V (1999) Est1 and Cdc13 as comediators of telomerase access. Science 286: 117–120. 4. Pennock E, Buckley K, Lundblad V (2001) Cdc13 delivers separate complexes to the telomere for end protection and replication. Cell 104: 387–396. 5. Bianchi A, Negrini S, Shore D (2004) Delivery of yeast telomerase to a DNA break depends on the recruitment functions of Cdc13 and Est1. Mol Cell 16: 139–146. 6. Chan A, Boulé JB, Zakian VA (2008) Two pathways recruit telomerase to Saccharomyces cerevisiaetelomeres. PLoS Genet 4: e1000236. doi:10.1371/journal.pgen.1000236. 7. Qi H, Zakian VA (2000) The Saccharomyces telomere-binding protein Cdc13p interacts with both the catalytic subunit of DNA polymerase α and the telomerase-associated Est1 protein. Genes Dev 14: 1777–1788. 8. Wellinger RJ, Wolf AJ, Zakian VA (1993) Saccharomyces telomeres acquire single-strand TG1-3 tails late in S phase. Cell 72: 51–60. 9. Dionne I, Wellinger RJ (1996) Cell cycle-regulated generation of single- stranded G-rich DNA in the absence of telomerase. Proc Natl Acad Sci USA 93: 13902–13907. 10. Larrivée M, LeBel C, Wellinger RJ (2004) The generation of proper constitutive G-tails on yeast telomeres is dependent on the MRX complex. Genes Dev 18: 1391–1396. 11. Bonetti D, Martina M, Clerici M, Lucchini G, Longhese MP (2009) Multiple pathways regulate 3′ overhang generation at S. cerevisiae telomeres. Mol Cell 35: 70–81. 12. Frank CJ, Hyde M, Greider CW (2006) Regulation of telomere elongation by the cyclin-dependent kinase CDK1. Mol Cell 24: 423–432. 13. Vodenicharov MD, Wellinger RJ (2006) DNA degradation at unprotected telomeres in yeast is regulated by the CDK1 (Cdc28/Clb) cell-cycle kinase. Mol Cell 24: 127–137. 14. Garvik B, Carson M, Hartwell L (1995) Single-stranded DNA arising at telomeres in cdc13 mutants may constitute a specific signal for the RAD9 checkpoint. Mol Cell Biol 15: 6128–6138. 87
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Contents
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Abstract
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Riassunto
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Introduction
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Introduction In Saccharomyces cerev
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Introduction Figure 3: A: Diagram s
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Introduction The Replication Protei
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Introduction Rap1 C terminal is ess
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Introduction and highest at G1 phas
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Introduction cleaves the RNA strand
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Introduction In the G1 cell cycle p
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Introduction impaired, and nuclease
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Introduction functionally compromis
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Introduction increasing the interac
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Introduction Similarly in fission y
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Introduction constitutive phosphory
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Shelterin-Like Proteins and Yku Inh
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Bonetti et. al., PLoS Genet. 2010 M
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Page 43 and 44: Bonetti et. al., PLoS Genet. 2010 M
Page 65 and 66: Rif1 Supports the Function of the C
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Page 102 and 103: Discussion The DNA damage proteins
Page 104 and 105: Discussion An hypothesis for the sp
Page 106 and 107: Discussion Figure 14: Model, part 2
Page 108 and 109: Telomeric G4 Discussion Apart from
Page 110 and 111: References 1. Hayflick L (1965) The
Page 112 and 113: References that is required for tel
Page 114 and 115: References 51. Surovtseva YV, Churi
Page 116 and 117: References telomere elongation in S
Page 118 and 119: References 100. Walker JR, Corpina
Page 120 and 121: References 124. Gravel S, Larrivée
Page 122 and 123: References 147. Lamarche BJ, Orazio
Page 124 and 125: References 170. Boltz KA, Leehy K,
Page 126 and 127: References 194. Nimonkar AV, Ozsoy
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