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NEW LC/MS METHOD FOR DETERMINATION OF PROGESTERONE IN HUMAN PLASMA …
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EIC 279.2 +MS2(315.2), Smoothed (0.3,1, GA)
EIC 279.2 +MS2(315.2), Smoothed (0.3,1, GA)
Figure 1. Chromatograms of a blank human male plasma (upper image) and a
plasma standard sample of 0.8 ng/ml PRG (RT 1.4 min)(lower image)
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+MS2(316.2), 1.1-1.1min #(274-276)
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+MS2(315.2), 1.1-1.1min #(273-277)
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Figure 2. Mass spectra of progesterone: MS/MS non-reactive spectrum - isolation
(upper image); MS/MS reactive spectrum - fragmentation (lower image)
The applied calibration curve model proved to be linear over the
concentration range 0.8 - 80 ng/ml PRG, with a determination coefficient
greater than 0.995. The mean calibration curve, y = a (±SD) x + b (±SD)
with SD standard deviation, was: y = 18097.2 (±1850.7) x – 1018.4 (±538.3),
N = 8 calibration points, n = 5 determinations for each calibration point. The
residuals had no tendency of variation with concentration and were between
±15% values.
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