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C. VARGA, M. MARIAN, A. PETER, D. BOLTEA, L. MIHALY-COZMUTA, E. NOUR
south–east of the Maramures County (see the map in Figure 6) and is a
non-metalliferous area due to the fact that there is no any mining and industrial
activity. Moreover, the village is at aproximatively 10 km away from the main
road, which is also considered a source of pollution. The concentrations of
the reference metal ions in soil are presented in Table 1.
We have collected P. vulgaris seeds out of the crop harvested in
Ferneziu and respectively in Oarta de Jos, in the fall of 2008.
Oarta de Jos
Figure 6. Map of Maramures County. Green circles identify the locations
from where we have collected the seeds of P. vulgaris
for the research interests of this paper.
Series of fifty seeds, collected in the above-mentioned areas, were
washed three times with distillated water and immersed in solutions with
different concentrations of heavy metal ions. The solutions containing the
mentioned metal ions were prepared using FeSO4, Pb(NO3)2, CuSO4, ZnSO4
salts. The initial concentrations of those solutions were established having in
view the same metal ions concentration in soil and are presented in Table 1.
Analyses on heavy metals
After 7 days of immersion at 22 0 C in the solution with ions of heavy
metals, we have washed three times the seeds with distillated water before
initiating our analyses, conducted in two ways.
1. We have relied on a German – made Krúss Optronic binocular
microscope in order to investigate the degree of imbibition by optical
microscopy. This method has entailed the use of a special reagent to color
each category of metal ions under scrutiny. Table 2 gives the color assigned
to each metal ion and corresponding to each reagent.