PDF file - Facultatea de Chimie şi Inginerie Chimică

More documents

Recommendations

Info

ELECTRICAL CHARACTERISTICS OF A BIOBATTERY WITH STAPHYLOCOCCUS AUREUS As we reduce the external resistance, a decrease of the voltage was recorded. In this way, we looked for having the smallest possible drop in voltage as the current is increased in order to attain the maximum power production over the investigated range of interest. The internal resistance of the battery and the maximum power generated were calculated by using the Mathworks MATLAB® environment for processing the experimental data. Spline functions have been used to approximate the power curve. (Figure 4). All of the equipment used was sterilized with hydrogen peroxide after the experiments. CONCLUSIONS The maximum power of the biobattery was of 17.57 µW, achieved at the internal resistance of 1.1 kΩ and for the external resistance of about 15 kΩ the minimum power reached was 5.45 µW, in the case of 0.05M substrate concentration in anode compartment. The value of the OCV remained at the constant value of about 0.56 V for a month. The difference which appears in OCV in both experiments can be explained by decreasing of the bacterial activity whit pH increase. The biobatteries represent a viable method of generating electricity from chemical energy. Further research must be done with the purpose of reducing the dimensions of the biobattery for possible applications in medicine and other fields. EXPERIMENTAL SECTION An electrochemical cell has been built with two chambers separated by a Nafion proton exchange membrane (Figure 1). Nine graphite rod electrodes of 6 mm in diameter and10 cm in length have been used for each chamber. For the anode compartment a 300 ml sodium acetate solution 0.05 M and 0.1 M respectively, has been combined with 2 ml of Staphylococcus aureus (10 35 9 cell/ml concentration). For the cathode compartment, 300 ml of 1 M FeCl3 solution was used. The circuits which contain a biobattery and multimeters were connected to a computer with continuous data acquisition software. The used bacteria were Staphylococcus aureus. The bacteria posed no threat to the working environment because they were non sporogenous. Bacterial strains. Both used bacterial strains, the UCLA 8076 (University of California, Los Angeles, USA) [16] and the 1190R (Semmelweis University, Budapest, Hungary) [17], were heterogeneous MRSA (methicillin-resistant Staphylococcus aureus)strains. These strains are preserved in glycerol (25% final concentration) at -80°C and prepared as follows: 5ml of overnight bacteria culture at 37°C in LB broth (Gibco BRL, Life Technologies, Paisley, Scotland)
C. CĂŢĂNAŞ, M. MOGOŞ, D. HORVAT, E. JAKAB, E. M. RUS, I. O. MARIAN were centrifuged in 15ml centrifuge tubes in a Sigma 1-18K centrifuge (Sigma Laborzentrifugen, Osterode am Harz, Germany –5,500 rpm, 10 min., room temperature) and the pellet was resuspended in 500μl sterile 50% glycerol. The thawed bacteria were cultured overnight in 5ml of Mueller Hinton broth (Fluka, Buchs, Switzerland) into a Certomat BS-T incubation shaker (Sartorius Stedim Biotech, Aubagne, France) at 37°C, 150 rpm until the culture reached an OD600 of 0.8-1.0 (Spekol UV VIS 3.02, Analytic Jena, Jena, Germany). A loop of bacterial suspension was passed on Mueller Hinton agar (Fluka, Buchs, Switzerland) to maintain the culture for further analysis. The bacteria were cultured also on blood agar plates (Fluka, Buchs, Switzerland) and confirmed using colony morphology method. REFERENCES 1. K.J. Chae, M. Choi, F.F. Ajayi, W. Park, I.S. Chang and I.S. Kim, Energy & Fuels, 2008, 22, 169. 2. B.H. Kim, I.S. Chang, G.M. Gadd, Appl. Microbiol. Biotechnol., 2007, 76, 485. 3. B.E. Logan, Water Science & Technology, 2005, 52, 31. 4. B.E. Rittmann et all., Environ. Sci. Tech., 2006, 15, 1097. 5. D.R. Lovley, Microbiology, 2006, 4, 497. 6. A.K. Shukla, P. Suresh, S. Berchmans, A. Rajendran, Current Science, 2004, 87, 455. 7. A.K. Marcus, C.I. Torres, B.E. Rittmann, Biotechnology and Bioengineering, 2007, 98, 1171. 8. K. Rabaey, W. Verstraete, TRENDS in Biotechnology, 2005, 23, 291. 9. S.E. Childers, S. Ciufo, D.R. Lovley, Nature, 2002, 416, 767. 10. A.K. Shukla, P. Suresh, S. Berchmans and A. Rajendran, Current Science, 2004, 87, 455. 11. T.H. Pham, J.K. Jang, I.S. Chang, and B.H. Kim, J. Microbiol. Biotechnol., 2004, 14, 324. 12. K.H. Kang, J.K. Jang, T.H. Pham, H.I. Moon, S.B. Chang, H. Kim, Biotechnol. Lett., 2003, 25, 1357. 13. I.S. Chang, J.K. Jang, G.C. Gil, M. Kim, H.J. Kim, B.W. Cho, Biosens. Bioelectron., 2004, 19, 607. 14. S. Cheng, H. Liu, B.E. Logan, Environ. Sci.Technol., 2006, 40, 364. 15. B.H. Kim, H.J. Kim, M.S. Hyun, D.H. Park, J. Microbiol.Biotechnol., 1999, 9, 127. 16. J.M. Swenson, F.C. Tenover, J. Clinical Microbiology, Cefoxitin Disk Study Group, 2005, 43(8), 3818. 17. L. Majoros, E. Papp Falusi, I. Andriko, F. Rozgonyi, Acta Microbiologica et Immunologica Hungarica, (1996) 43(2-3):160. 36
Page 1 and 2: R /(mol m -2 s -1 ) 0.06 0.05 0.04
Page 3 and 4: ANA-MARIA CORMOS, JOZSEF GASPAR, AN
Page 5 and 6: Studia Universitatis Babes-Bolyai C
Page 7 and 8: 6 PROFESSOR IOAN BÂLDEA AT HIS 70
Page 9 and 10: MARCELA ACHIM, DANA MUNTEAN, LAURIA
Page 15 and 16: STUDIA UNIVERSITATIS BABEŞ-BOLYAI,
Page 17 and 18: STUDIES ON WO3 THIN FILMS PREPARED
Page 25 and 26: PREPARATION AND CHARACTERIZATION OF
Page 31 and 32: 32 C. CĂŢĂNAŞ, M. MOGOŞ, D. HO
Page 33: 34 C. CĂŢĂNAŞ, M. MOGOŞ, D. HO
Page 37 and 38: 38 ANA-MARIA CORMOS, JOZSEF GASPAR,
Page 39 and 40: ANA-MARIA CORMOS, JOZSEF GASPAR, AN
Page 49 and 50: 50 EUGEN DARVASI, LADISLAU KÉKEDY-
Page 61 and 62: MATHEMATICAL MODELING FOR THE CRYST
Page 71 and 72: STUDY OF THE CHROMATOGRAPHIC RETENT
Page 81 and 82: LOWER RIM SILYL SUBSTITUTED CALIX[8
Page 83 and 84: LOWER RIM SILYL SUBSTITUTED CALIX[8
Page 85 and 86:
LOWER RIM SILYL SUBSTITUTED CALIX[8
Page 87 and 88:
STUDIA UNIVERSITATIS BABEŞ-BOLYAI,
Page 89 and 90:
THE INTERACTION OF SILVER NANOPARTI
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
OPTIMISATION OF COPPER REMOVAL FROM
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
MELINDA-HAYDEE KOVACS, DUMITRU RIST
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
IRON DOPED CARBON AEROGEL AS CATALY
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
128 ANDRADA MĂICĂNEANU, HOREA BED
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
ANDRADA MĂICĂNEANU, HOREA BEDELEA
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
CRISTINA MIHALI, GABRIELA OPREA, EL
Page 139 and 140:
144 CRISTINA MIHALI, GABRIELA OPREA
Page 141 and 142:
146 Interfering ion, J - I - DBS -
Page 143 and 144:
CONCLUSIONS 148 CRISTINA MIHALI, GA
Page 145 and 146:
150 CRISTINA MIHALI, GABRIELA OPREA
Page 147 and 148:
152 D. MIHU, L. VLASE, S. IMRE, C.
Page 149 and 150:
154 Intens. x105 1.5 1.0 0.5 0.0 x1
Page 151 and 152:
D. MIHU, L. VLASE, S. IMRE, C. M. M
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
162 A. PETER, M. BAIA, F. TODERAS,
Page 159 and 160:
164 (a) V relative [%] (c) V relati
Page 161 and 162:
A. PETER, M. BAIA, F. TODERAS, M. L
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
BIOSORPTION OF PHENOL FROM AQUEOUS
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
186 ANDREI ROTARU, MIHAI GOŞA, EUG
Page 181 and 182:
Page 183 and 184:
ANDREI ROTARU, MIHAI GOŞA, EUGEN S
Page 185 and 186:
Page 187 and 188:
194 OCTAVIAN STAICU, VALENTIN MUNTE
Page 189 and 190:
ln(τ i / s) OCTAVIAN STAICU, VALEN
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
204 MARIA ŞTEFAN, IOAN BÂLDEA, RO
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
EXPERIMENTAL SECTION 210 MARIA ŞTE
Page 205 and 206:
Page 207 and 208:
EQUILIBRIUM STUDY ON ADSORPTION PRO
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
STRATEGIES OF HEAVY METAL UPTAKE BY
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
CORROSION INHIBITION OF BRONZE BY A
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Page 241 and 242:
E / mV vs. SCE POTASSIUM-SELECTIVE
Page 243 and 244:
POTASSIUM-SELECTIVE ELECTRODE BASED
Page 245 and 246:
POTASSIUM-SELECTIVE ELECTRODE BASED
Page 247 and 248:
256 CODRUTA VARODI, DELIA GLIGOR, L
Page 249 and 250:
CODRUTA VARODI, DELIA GLIGOR, LEVEN
Page 251 and 252:
Page 253 and 254:
Page 255 and 256:
Page 257 and 258:
PHARMACOKINETIC INTERACTION BETWEEN
Page 259 and 260:
Page 261:
show all

PDF file - Facultatea de Chimie şi Inginerie Chimică

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?