Muscarinic M1, M3, Nicotinic,GABAA and GABAB Receptor ...

Studies using [ 3 H] Radioligands 50
Binding studies in the Brain regions 50
Total muscarinic, muscarinic M1 and M3 receptor
Binding studies 50
Total GABA receptor binding studies 51
Protein determination 51
Analysis of the Receptor Binding Data 52
Linear regression analysis for Scatchard plots 52
Gene expression studies in Different Brain Regions and
Pancreas of control and Experimental rats 52
Isolation of RNA 52
REAL-TIME Polymerase Chain Reaction 53
cDNA synthesis 53
Real-Time PCR assays 53
Immunohistochemistry of Muscarinic M1, M3, α7 nicotinic
acetylcholine and GABAAα1 Receptors in the brain regions of
control and experimental rats using confocal microscope 55
Immunohistochemistry of Muscarinic M1, M3 and GABAAα1
Receptors in the pancreas of control and experimental
rats using confocal microscope 55
STATISTICS 57
RESULTS 58
Body Weight and Blood Glucose Level 58
Circulating insulin level 58
Behavioural studies 59