POTASSIUM CHLORIDE CAS N°: 7447-40-7

OECD SIDS
POTASSIUM CHLORIDE
Date: 30-MAR-2003
4. Ecotoxicity Substance ID: 7447-40-7
AQUATIC ORGANISMS
4.1 Acute/Prolonged Toxicity to Fish
Type of test: static
Species:
Pimephales promelas (Fathead minnows)
Exposure period: 24, 48, 96 hours
Unit: mg/l Analytical monitoring: yes
LC50/24 hours: = 950
LC50/48 hours: = 910
LC50/96 hours: = 880
Method:
other: EPA/600/4-90/027
Year: 1997 GLP: no
Test substance: as prescribed by 1.1-1.4; reagent grade
Remarks:
All organisms, 1 to 7 days old, were obtained from
in-house cultures. Brood stock were cultured at
20-25 degrees C in tap water pretreated with
activated carbon. Larva were fed Artemia sp. twice
daily until they were used in testing.
The toxicity tests followed the general guidance
of the U.S. Environmental Protection Agency. All
tests were conducted in 30-ml plastic beakers
containing 10 ml test solution and five organisms
per chamber. Tests were conducted under a 16-h:8-h
light-dark photoperiod at 25 degrees C.
Dilution/control water for all tests was
moderately hard reconstituted water (MHRW).
Exposure period was 96 hours, with daily
observations of mortality. The criteria for death
were no visible movement and no response to
prodding. 100 µl of concentrated brine shrimp
nauplii was added after 48 hours of exposure.
Test solutions were prepared by dissolving 10,000
mg/l KCl salt in MHRW, and diluting with MHRW to a
series of 5 test concentrations spaced on 0.5
dilution factor. As testing proceeded, test
concentrations were spaced much more closely to
better define responses near the effect threshold.
All ion concentrations measured in stock solutions
were compared to nominal values. If measured
concentration differed from the nominal value by
more than 20 %, the actual measured concentrations
were substituted for the nominal concentrations.
Background KCl content in the dilution water MHRW
were added to the calculated contributions from
the stock solutions.
Dissolved oxygen (DO) and pH were measured in
selected test solutions during actual toxicity
testing: Measured DO concentrations were always >
40 % saturation; measured pH was between 7.5-9.0.
3 replicate tests were conducted, and average LC50
values were calculated as the arithmetic mean of
the values; SD = 15, range 1.4-62.
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