POTASSIUM CHLORIDE CAS N°: 7447-40-7

OECD SIDS POTASSIUM CHLORIDE
Date: 30-MAR-2003
5. Toxicity Substance ID: 7447-40-7
activation: With Aroclor 1254 induced Fisher rat liver S9
homogenate or with noninduced Fisher 344 rat liver
S9, without activation
Results:
With metabolic activation (Aroclor 1254 induced):
positive at 5000 µg/ml. With metabolic activation
(noninduced): positive at 4000 and 5000 µg/ml.
Without metabolic activation: negative up to 5000
µg/ml, however, higher, concentrations up to 8000
µg/ml, appeared to be toxic and mutagenic.
Method: OECD 476
Year: 1988 GLP: no data
Test substance:
Remarks:
as prescribed by 1.1-1.4, purity: commercial grade
Media: Fischer's liquid medium for leucemic
cells supplemented with 10 % horse serum. Positive
control was Ethyl methanesulfonate or 3-
methylcholantrene and was positive under all test
conditions. Negative control was 10 % water and
solvent for the test chemical, and was negative
under all test conditions. Treatment up to 5000
µg/ml was not toxic, and did not reduce the
relative total growth below 50-65 %.
Source:
Norsk Hydro ASA
Reliability: (1) reliable without restriction
Flag:
Key study for SIDS
01-MAR-2001 (55)
Type:
mammalian cell gene mutation test
System of
testing: mouse lymphoma cell (L5178Y), TK +/- heterozygote
Concentration: 0-5000 µg/ml
Metabolic
activation: With Aroclor 1254 induced male Fisher 344 rats S9
liver homogenate. Without activation
Results:
With metabolic activation: In the first trial the
relative total growth decreased with increased
concentrations up to 4000 µg/ml, and no
mutagenicity was reported. The second trial yielded
a positive mutagenic response with a twofold
increase at 4000 µg/ml and a 2.5 fold increase at
5000 µg/ml. The third trial yielded a
concentration-dependent increase in mutations of
about twofold at 3645 µg/ml, and threefold at 4050
µg/ml. Overall the test with S9 activation was
evaluated as positive.
Without metabolic activation: negative up to 5000
µg/ml in the first trial. In the second trial
mutations was increased 2.5 fold from 1049 to 3200
µg/ml, however, the increase was not dose-related.
In the third trial cytotoxicity with no
mutagenicity was reported. Overall the results
without S9 activation were evaluated as negative.
Method: OECD 476
Year: 1988 GLP: no data
Test substance: as prescribed by 1.1-1.4, purity: commercial grade
Remarks:
Media: Fischer's liquid medium for leucemic cells
supplemented with 10 % horse serum. Positive
control was Ethyl methanesulfonate or 3-
methylcholantrene and was positive under all test
conditions. Negative control was 10 % water and
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