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Institute for Sanitary Engineering, Water Quality and Solid Waste ...

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However, the simultaneous degradation of mixtures of<br />

methyl substituted or halogene substituted aromatics<br />

is sophisticated because of incompatible induced pathways<br />

<strong>and</strong> the production of toxic intermediates as a<br />

result of this (so called suicide inactivation). Furthermore,<br />

based on regulatory effects only one specific pathway<br />

<strong>for</strong> degradation of single compounds is induced<br />

leading to non simultaneous degradation of the other<br />

waste air compounds.<br />

In context of this work two bacterial strains Burkholderia<br />

fungorum FLU 100 <strong>and</strong> Rhodococcus wratislaviensis<br />

OCT 10 were analysed in detail. Next to a interesting<br />

spectrum of degradable aromatic substrates,<br />

both bacteria show novel, non described pathways.<br />

FLU 100 is capable to mineralise toluene using a modified<br />

ortho-pathway <strong>and</strong> OCT 10 is able to degrade the<br />

persistent compound 2-chlorotoluene using a so called<br />

meta-pathway. Studies of feasibility of simultaneous<br />

degradation of toluene <strong>and</strong> 2-chlorotoluene were extended<br />

by further aromatic compounds like fluorobenzene,<br />

chlorobenzene, benzene <strong>and</strong> o-xylene. The<br />

studies were operated in laboratory <strong>and</strong> half-technical<br />

scale. The latter were realised in a biotrickling filter<br />

system where the effect of highly fluctuating waste air<br />

Flow chart of a biotricklingfilter system with filtration, humidification <strong>and</strong> solvent dosage as<br />

preinstalled units <strong>for</strong> biological treatment of a ternary mixture of 2-chlorotoluene, o-xylene <strong>and</strong><br />

toluene by the bacterial strain Rhodococcus wratislaviensis OCT 10.<br />

67

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