Plant Variety Journal - IP Australia

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Plant Varieties Journal Vol. 23 Number 4 Details of Application Application Number 2008/274 Variety Name ‘Riverina Alan’ Genus Species Lavandula x intermedia Common Name Lavandin Synonym Nil Accepted Date 15 Dec 2008 Applicant Charles Sturt University Agent N/A Qualified Person Nigel Urwin,Charles Sturt University, Wagga Wagga, NSW. Details of Comparative Trial Location Charles Sturt University Descriptor Lavandula (Lavandula) TG/194/1 Period Sep 2009-Dec 2011 Conditions All plants were propagated by Larkman Nurseries, Lilydale, Melbourne and provided in 50x75mm tubes. All plants were potted into 9cm diameter pots in Debco TM Terracotta and Tub potting mix. Plants were watered every other day by hand. Plants were repotted into 25cm diameter pots in the same growing medium late August 2010. Plants were then watered by automatic overhead sprinklers and occasionally by hand. Osmocote TM slow release fertiliser and fungicides were applied occasionally as required. Trial Design The trial consisted of 8 L. x intermedia ‘Hidcote Giant’, 10 L. x intermedia ‘Impress Purple’, 10 L. x intermedia ‘Seal’, 9 L. x intermedia ‘Grosso’ 10 L. hybrid ‘Riverina Thomas’ and 10 L. hybrid ‘Riverina Alan’. Plants were arranged in a completely randomised block design (10x6). The trial was designed for DUS analysis of two varieties the other being Riverina Thomas was included as a comparator also. Measurements Observations were made on 13 Dec 2010, mid-flowering and early Oct before flowering for observation on plant size and form. Corolla colour was N88B on all varieties. RHS Chart - edition Fifth edition Origin and Breeding Induced mutation: The starting material was L. x intermedia ‘Seal’. ‘Seal’ is common variety of L. x intermedia. Tissue culture techniques were used to initiate and establish shoot cultures of ‘Seal’. Cultured shoots were treated with colchicine to induce polyploidy and transferred to media to induce root formation. Surviving explants which formed roots were acclimatised to glass house conditions and potted up. Plants were placed in the lavender collection at Charles Sturt University and were grown to flowering under conditions which allowed open pollination. All known L. x intermedia varieties are infertile hybrids of L. angustifolia and L. latifolia. They occur naturally in the wild and some have been bred. Consequently they do not produce seed. Often conversion of a diploid sterile hybrid to tetraploid results in restoration of fertility and seed production in other genera. It was anticipated that similar conversion of L. x intermedia ‘Seal’ to tetraploidy status would restore fertility. To detect tetraploids we therefore attempted to collect seed from the ‘Seal’ plants surviving colchicine treatments. A number of plants which had colchicine treatments produced seed whereas none of the controls plants treated with water rather than colchicine produced any seed. Ten seeds were obtained Page 148 of 322
Plant Varieties Journal Vol. 23 Number 4 from a single colchicine treated ‘Seal’ plant. Seeds were germinated in a Petri-dish on filter paper soaked in gibberellic acid to induce germination. Nine of the seeds germinated and plants were potted up and grown until flowering. The plants were grown under the same conditions as the parent plants above. Cuttings from individual plants were propagated and planted in the field collection to observe their morphology and performance. Between the nine plants grown some morphological variation was seen, however, most were more vigorous and much larger plants than the parent variety ‘Seal’. The plant with the greatest vigour and largest flowers was selected. This plant was designated ‘Riverina Alan’ or CSU138. All plants called ‘Riverina Alan’ were clonally propagated as cuttings from the one plant. Characteristics of L. x intermedia ‘Riverina Alan’ are that it is a larger plant than ‘Seal’ with larger leaves and flowers. It has thicker peduncles and stems, it retains the open form of ‘Seal’ with long peduncles but flowers slightly earlier than Seal. From chromosome number estimates and flow cytometry analysis of nuclear DNA content ‘Riverina Alan’ and the other ‘Seal’ derived seedlings are approximately triploid whereas the parent ‘Seal’ was diploid. ‘Riverina Alan’ is infertile and has not produced any seed tested over two seasons in the collection at CSU. In summary, the diploid L. x intermedia Seal (infertile) was converted to a tetraploid (fertile). Following open pollination of the tetraploid seeds were collected from these and ‘Riverina Alan’ is a seedling selected on the basis of size of whole plant and various plant organs. Choice of Comparators Characteristics used for grouping varieties to identify the most similar Variety of Common Knowledge Organ/Plant Part Context State of Expression in Group of Varieties Plant size medium Most Similar Varieties of Common Knowledge identified (VCK) Name Comments ‘Seal’ Parent. ‘Hidcote Giant’ ‘Impress Purple’ ‘Grosso’ ‘Riverina Thomas’ Variety Description and Distinctness - Characteristics which distinguish the candidate from one or more of the comparators are marked with a tick. Organ/Plant Part: ‘Riverina ‘Hidcote ‘Impress ‘Riverina ‘Grosso’ Context Alan’ Giant’ Purple’ Thomas’ *Plant: growth habit ‘Seal’ bushy globular globular globular globular bushy *Plant: size medium medium medium medium medium medium Plant: intensity of green colour of foliage Plant: intensity of grey tinge of foliage *Plant: attitude of outer flowering stems medium medium medium medium medium medium medium to strong medium to strong medium to strong medium to strong medium to strong erect semi-erect erect semi-erect semi-erect erect medium to strong Page 149 of 322
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